Alternative splicing in the Caenorhabditis elegans DNA topoisomerase I gene

Myon Hee Lee, Yeon Joo Jang, Hyeon Sook Koo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

5'-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5'-terminus of the cDNA sequence suggested trans-splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1396
Issue number2
DOIs
Publication statusPublished - 1998 Mar 9

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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