5'-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5'-terminus of the cDNA sequence suggested trans-splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.
|Number of pages||8|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|Publication status||Published - 1998 Mar 9|
Bibliographical noteFunding Information:
We are grateful to Dr. Chris Link (University of Colorado) for sending us a C. elegans genomic DNA library and to Dr. Alan Coulson (Medical Research Council, UK) for several cosmid clones of the C. elegans genomic DNA. This work was supported by a genetic engineering research grant from the Korean Ministry of Education and a research grant from Yonsei University.
All Science Journal Classification (ASJC) codes
- Structural Biology