Alternative splicing in the Caenorhabditis elegans DNA topoisomerase I gene

Myon Hee Lee, Yeon Joo Jang, Hyeon-Sook Koo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

5'-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5'-terminus of the cDNA sequence suggested trans-splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1396
Issue number2
DOIs
Publication statusPublished - 1998 Mar 9

Fingerprint

Type I DNA Topoisomerase
Caenorhabditis elegans
Alternative Splicing
Complementary DNA
Genes
Exons
Messenger RNA
Spliced Leader RNA
Trans-Splicing
Polymerase chain reaction
RNA Precursors
Transcription
Reverse Transcription
Amplification
Embryonic Structures
Clone Cells
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

@article{47542c82036a4a27a1485826f94f1ed2,
title = "Alternative splicing in the Caenorhabditis elegans DNA topoisomerase I gene",
abstract = "5'-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5'-terminus of the cDNA sequence suggested trans-splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.",
author = "Lee, {Myon Hee} and Jang, {Yeon Joo} and Hyeon-Sook Koo",
year = "1998",
month = "3",
day = "9",
doi = "10.1016/S0167-4781(97)00209-1",
language = "English",
volume = "1396",
pages = "207--214",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "2",

}

Alternative splicing in the Caenorhabditis elegans DNA topoisomerase I gene. / Lee, Myon Hee; Jang, Yeon Joo; Koo, Hyeon-Sook.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1396, No. 2, 09.03.1998, p. 207-214.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alternative splicing in the Caenorhabditis elegans DNA topoisomerase I gene

AU - Lee, Myon Hee

AU - Jang, Yeon Joo

AU - Koo, Hyeon-Sook

PY - 1998/3/9

Y1 - 1998/3/9

N2 - 5'-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5'-terminus of the cDNA sequence suggested trans-splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.

AB - 5'-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5'-terminus of the cDNA sequence suggested trans-splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.

UR - http://www.scopus.com/inward/record.url?scp=0032498839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032498839&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(97)00209-1

DO - 10.1016/S0167-4781(97)00209-1

M3 - Article

C2 - 9540836

AN - SCOPUS:0032498839

VL - 1396

SP - 207

EP - 214

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 2

ER -