Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer

Eun Ju Lee, Minwha Jo, Junsoo Park, Wei Zhang, Je Ho Lee

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The two previously identified major splice variants of interferon regulatory factor 1 (IRF-1) do not appear to affect IRF-1-mediated gene activation. We searched for additional splice variants and examined their effect on wild-type IRF-1. RT-PCR experiments using normal and malignant human cervical tissue samples revealed five variants lacking some combination of exons 7, 8, and 9; their expression levels were higher in the malignant samples. These variants had predicted deletions of the functional domain or truncated protein isoforms, had different transcriptional activities, and attenuated transcriptional activity of IRF-1. Unlike the cell cycle-dependent IRF-1 transcript, the splice variant mRNA levels remained consistent throughout the cell cycle. The variant proteins were more stable than the IRF-1 protein, which may explain the strong inhibition of IRF-1 transcription in the presence of relatively small quantities of the alternative transcripts. In conclusion, alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.

Original languageEnglish
Pages (from-to)882-888
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume347
Issue number4
DOIs
Publication statusPublished - 2006 Sep 8

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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