Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer

Eun Ju Lee, Minwha Jo, Junsoo Park, Wei Zhang, Je Ho Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The two previously identified major splice variants of interferon regulatory factor 1 (IRF-1) do not appear to affect IRF-1-mediated gene activation. We searched for additional splice variants and examined their effect on wild-type IRF-1. RT-PCR experiments using normal and malignant human cervical tissue samples revealed five variants lacking some combination of exons 7, 8, and 9; their expression levels were higher in the malignant samples. These variants had predicted deletions of the functional domain or truncated protein isoforms, had different transcriptional activities, and attenuated transcriptional activity of IRF-1. Unlike the cell cycle-dependent IRF-1 transcript, the splice variant mRNA levels remained consistent throughout the cell cycle. The variant proteins were more stable than the IRF-1 protein, which may explain the strong inhibition of IRF-1 transcription in the presence of relatively small quantities of the alternative transcripts. In conclusion, alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.

Original languageEnglish
Pages (from-to)882-888
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume347
Issue number4
DOIs
Publication statusPublished - 2006 Sep 8

Fingerprint

Interferon Regulatory Factor-1
Alternative Splicing
Uterine Cervical Neoplasms
Exons
Cell Cycle
Cells
Transcription
Transcriptional Activation
Protein Isoforms
Proteins
Genes
Chemical activation
Tissue
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{797b9d87d9f44fd995eb89a0e705c68b,
title = "Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer",
abstract = "The two previously identified major splice variants of interferon regulatory factor 1 (IRF-1) do not appear to affect IRF-1-mediated gene activation. We searched for additional splice variants and examined their effect on wild-type IRF-1. RT-PCR experiments using normal and malignant human cervical tissue samples revealed five variants lacking some combination of exons 7, 8, and 9; their expression levels were higher in the malignant samples. These variants had predicted deletions of the functional domain or truncated protein isoforms, had different transcriptional activities, and attenuated transcriptional activity of IRF-1. Unlike the cell cycle-dependent IRF-1 transcript, the splice variant mRNA levels remained consistent throughout the cell cycle. The variant proteins were more stable than the IRF-1 protein, which may explain the strong inhibition of IRF-1 transcription in the presence of relatively small quantities of the alternative transcripts. In conclusion, alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.",
author = "Lee, {Eun Ju} and Minwha Jo and Junsoo Park and Wei Zhang and Lee, {Je Ho}",
year = "2006",
month = "9",
day = "8",
doi = "10.1016/j.bbrc.2006.06.145",
language = "English",
volume = "347",
pages = "882--888",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer. / Lee, Eun Ju; Jo, Minwha; Park, Junsoo; Zhang, Wei; Lee, Je Ho.

In: Biochemical and Biophysical Research Communications, Vol. 347, No. 4, 08.09.2006, p. 882-888.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer

AU - Lee, Eun Ju

AU - Jo, Minwha

AU - Park, Junsoo

AU - Zhang, Wei

AU - Lee, Je Ho

PY - 2006/9/8

Y1 - 2006/9/8

N2 - The two previously identified major splice variants of interferon regulatory factor 1 (IRF-1) do not appear to affect IRF-1-mediated gene activation. We searched for additional splice variants and examined their effect on wild-type IRF-1. RT-PCR experiments using normal and malignant human cervical tissue samples revealed five variants lacking some combination of exons 7, 8, and 9; their expression levels were higher in the malignant samples. These variants had predicted deletions of the functional domain or truncated protein isoforms, had different transcriptional activities, and attenuated transcriptional activity of IRF-1. Unlike the cell cycle-dependent IRF-1 transcript, the splice variant mRNA levels remained consistent throughout the cell cycle. The variant proteins were more stable than the IRF-1 protein, which may explain the strong inhibition of IRF-1 transcription in the presence of relatively small quantities of the alternative transcripts. In conclusion, alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.

AB - The two previously identified major splice variants of interferon regulatory factor 1 (IRF-1) do not appear to affect IRF-1-mediated gene activation. We searched for additional splice variants and examined their effect on wild-type IRF-1. RT-PCR experiments using normal and malignant human cervical tissue samples revealed five variants lacking some combination of exons 7, 8, and 9; their expression levels were higher in the malignant samples. These variants had predicted deletions of the functional domain or truncated protein isoforms, had different transcriptional activities, and attenuated transcriptional activity of IRF-1. Unlike the cell cycle-dependent IRF-1 transcript, the splice variant mRNA levels remained consistent throughout the cell cycle. The variant proteins were more stable than the IRF-1 protein, which may explain the strong inhibition of IRF-1 transcription in the presence of relatively small quantities of the alternative transcripts. In conclusion, alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.

UR - http://www.scopus.com/inward/record.url?scp=33746339222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746339222&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.06.145

DO - 10.1016/j.bbrc.2006.06.145

M3 - Article

C2 - 16857162

AN - SCOPUS:33746339222

VL - 347

SP - 882

EP - 888

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -