Alveolar bone resorption induced by CD4 + CD45RB high-density T-cell transfer in immunocompromised mice

Jung Chul Park, Bo Kyoung Kim, Im Hee Jung, Eunyoung Choi, ChangSung Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB highdensity) T-cell (CD4 + CD45RB High T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals. Methods: CD4 + CD45RB High and CD4 + CD45RB Low (antigenexperienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease- like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated. Results: Histologic and micro-CT analysis revealed that the transfer of CD4 + CD45RB High T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/ adipogenic potential of ABCs. Furthermore, it was found that CD4 + CD45RB High T-cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model. Conclusion: CD4 + CD45RB High T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.

Original languageEnglish
JournalJournal of Periodontology
Volume85
Issue number9
DOIs
Publication statusPublished - 2014 Jan 1

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Alveolar Bone Loss
Bone Resorption
Alveolar Epithelial Cells
T-Lymphocytes
Mesenchymal Stromal Cells
Periodontal Ligament
Severe Combined Immunodeficiency
X-Ray Microtomography
Stem Cells
Dental Cementum
Transfer (Psychology)
T-Lymphocyte Subsets
Stem Cell Transplantation
Tibia
Mandible
Inflammatory Bowel Diseases
Down-Regulation
Antigens

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

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title = "Alveolar bone resorption induced by CD4 + CD45RB high-density T-cell transfer in immunocompromised mice",
abstract = "Background: The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB highdensity) T-cell (CD4 + CD45RB High T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals. Methods: CD4 + CD45RB High and CD4 + CD45RB Low (antigenexperienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease- like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated. Results: Histologic and micro-CT analysis revealed that the transfer of CD4 + CD45RB High T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/ adipogenic potential of ABCs. Furthermore, it was found that CD4 + CD45RB High T-cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model. Conclusion: CD4 + CD45RB High T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.",
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Alveolar bone resorption induced by CD4 + CD45RB high-density T-cell transfer in immunocompromised mice . / Park, Jung Chul; Kim, Bo Kyoung; Jung, Im Hee; Choi, Eunyoung; Kim, ChangSung.

In: Journal of Periodontology, Vol. 85, No. 9, 01.01.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alveolar bone resorption induced by CD4 + CD45RB high-density T-cell transfer in immunocompromised mice

AU - Park, Jung Chul

AU - Kim, Bo Kyoung

AU - Jung, Im Hee

AU - Choi, Eunyoung

AU - Kim, ChangSung

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB highdensity) T-cell (CD4 + CD45RB High T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals. Methods: CD4 + CD45RB High and CD4 + CD45RB Low (antigenexperienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease- like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated. Results: Histologic and micro-CT analysis revealed that the transfer of CD4 + CD45RB High T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/ adipogenic potential of ABCs. Furthermore, it was found that CD4 + CD45RB High T-cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model. Conclusion: CD4 + CD45RB High T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.

AB - Background: The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB highdensity) T-cell (CD4 + CD45RB High T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals. Methods: CD4 + CD45RB High and CD4 + CD45RB Low (antigenexperienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease- like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated. Results: Histologic and micro-CT analysis revealed that the transfer of CD4 + CD45RB High T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/ adipogenic potential of ABCs. Furthermore, it was found that CD4 + CD45RB High T-cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model. Conclusion: CD4 + CD45RB High T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.

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U2 - 10.1902/jop.2014.140077

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M3 - Article

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JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

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