Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-α Pathway

Joo Young Kim, Jung Ho Sohn, Je Min Choi, Jae Hyun Lee, Chein Soo Hong, Joo Shil Lee, Jung Won Park

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The activity of the serine protease in the German cockroach allergen is important to the development of allergic disease. The protease-activated receptor (PAR)-2, which is expressed in numerous cell types in lung tissue, is known to mediate the cellular events caused by inhaled serine protease. Alveolar macrophages express PAR-2 and produce considerable amounts of tumor necrosis factor (TNF)-α. We determined whether the serine protease in German cockroach extract (GCE) enhances TNF-α production by alveolar macrophages through the PAR-2 pathway and whether the TNF-α production affects GCE-induced pulmonary inflammation. Effects of GCE on alveolar macrophages and TNF-α production were evaluated using in vitro MH-S and RAW264.6 cells and in vivo GCE-induced asthma models of BALB/c mice. GCE contained a large amount of serine protease. In the MH-S and RAW264.7 cells, GCE activated PAR-2 and thereby produced TNF-α. In the GCE-induced asthma model, intranasal administration of GCE increased airway hyperresponsiveness (AHR), inflammatory cell infiltration, productions of serum immunoglobulin E, interleukin (IL)-5, IL-13 and TNF-α production in alveolar macrophages. Blockade of serine proteases prevented the development of GCE induced allergic pathologies. TNF-α blockade also prevented the development of such asthma-like lesions. Depletion of alveolar macrophages reduced AHR and intracellular TNF-α level in pulmonary cell populations in the GCE-induced asthma model. These results suggest that serine protease from GCE affects asthma through an alveolar macrophage and TNF-α dependent manner, reflecting the close relation of innate and adaptive immune response in allergic asthma model.

Original languageEnglish
Article numbere47971
JournalPloS one
Volume7
Issue number10
DOIs
Publication statusPublished - 2012 Oct 19

Fingerprint

Blattellidae
Cockroaches
Blattella germanica
tumor necrosis factors
Alveolar Macrophages
Blattodea
macrophages
Tumor Necrosis Factor-alpha
inflammation
Serine Proteases
Inflammation
PAR-2 Receptor
asthma
serine proteinases
extracts
Asthma
production economics
proteinases
receptors
lungs

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Kim, Joo Young ; Sohn, Jung Ho ; Choi, Je Min ; Lee, Jae Hyun ; Hong, Chein Soo ; Lee, Joo Shil ; Park, Jung Won. / Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-α Pathway. In: PloS one. 2012 ; Vol. 7, No. 10.
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Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-α Pathway. / Kim, Joo Young; Sohn, Jung Ho; Choi, Je Min; Lee, Jae Hyun; Hong, Chein Soo; Lee, Joo Shil; Park, Jung Won.

In: PloS one, Vol. 7, No. 10, e47971, 19.10.2012.

Research output: Contribution to journalArticle

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