Amadori-glycated albumin-induced vascular smooth muscle cell proliferation and expression of inhibitor of apoptosis protein-1 and nerve growth factor-γ

byungwan lee, Jahei Ihm, Jun Gu Kang, Moon Gi Choi, Hyung Joon Yoo, Sung Hee Ihm

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We investigated the effects of Amadori-glycated serum albumin (GSA) on cell proliferation as well as expressions of antioxidant enzyme genes and marker genes associated with signal transduction pathways in rat aortic vascular smooth muscle cells (VSMCs). Quiescent VSMCs treated with GSA (0-500 μg/mL, 48 h) exhibited a dose-dependent increase in proliferation that was prevented by PD98059 (25 μM), suggesting a MAPK-dependent signaling pathway. Compared with bovine serum albumin (BSA)-treated cells, the GSA (500 μg/mL, 24~h)-treated VSMCs showed a higher superoxide dismutase 2 gene expression in quantitative RT-PCR, suggesting the involvement of oxidative stress. In a focused oligonucleotide array containing 96 signal transduction-related genes, expression of inhibitor of apoptosis protein-1 (IAP-1), nerve growth factor-γ (NGF-γ), and c-jun genes was significantly higher in the GSA-treated VSMCs. These results suggest that induction of antiapoptotic proteins like IAP-1 and strong mitogens like NGF-γ by GSA might further contribute to the VSMC proliferation and accelerated vascular remodeling in diabetes.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalBioFactors
Volume31
Issue number3-4
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Inhibitor of Apoptosis Proteins
Cell proliferation
Nerve Growth Factor
Vascular Smooth Muscle
Serum Albumin
Smooth Muscle Myocytes
Muscle
Cell Proliferation
Cells
Signal transduction
Gene expression
Signal Transduction
Genes
jun Genes
Gene Expression
Oxidative stress
Medical problems
Bovine Serum Albumin
Oligonucleotide Array Sequence Analysis
Mitogens

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

Cite this

lee, byungwan ; Ihm, Jahei ; Kang, Jun Gu ; Choi, Moon Gi ; Yoo, Hyung Joon ; Ihm, Sung Hee. / Amadori-glycated albumin-induced vascular smooth muscle cell proliferation and expression of inhibitor of apoptosis protein-1 and nerve growth factor-γ. In: BioFactors. 2007 ; Vol. 31, No. 3-4. pp. 145-153.
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Amadori-glycated albumin-induced vascular smooth muscle cell proliferation and expression of inhibitor of apoptosis protein-1 and nerve growth factor-γ. / lee, byungwan; Ihm, Jahei; Kang, Jun Gu; Choi, Moon Gi; Yoo, Hyung Joon; Ihm, Sung Hee.

In: BioFactors, Vol. 31, No. 3-4, 01.01.2007, p. 145-153.

Research output: Contribution to journalArticle

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AU - Yoo, Hyung Joon

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