Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96: Its pathological implications in lupus-like autoimmune diseases

Jung Min Han, Gyu Park Sang, Bei Liu, Bum Joon Park, Young Kim Jin, He Jin Cheng, Wook Song Yeong, Zihai Li, Sunghoon Kim

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Aminoacyl-tRNA synthetase-interacting multifunctional protein 1 (AIMP1; previously known as p43) is a multifunctional protein that was initially found in multitRNA synthetase complex. In the present study, screening of the AIMP1-binding proteins revealed that AIMP1 can form a molecular complex with heat shock protein gp96. AIMP1 enhances gp96 dimerization and the interaction between gp96 and KDEL receptor-1 (KDELR-1), which mediates the retrieval of KDEL-containing proteins from Golgi to the endoplasmic reticulum (ER). The interaction between gp96 and KDELR-1 was reduced in AIMP1-deficient cells, and this disturbed ER retention of gp96 and increased its cell surface localization. Moreover, this localization of gp96 at the cell surface was suppressed by its interaction with AIMP1 and enhanced by the depletion of endogenous AIMP1. In addition, AIMP1-deficient mice showed dendritic cell activation attributable to increased gp96 surface presentation and lupus-like autoimmune phenotypes. These results suggest that AIMP1 acts as a regulator of the ER retention of gp96 and provide a new perspective of the regulatory mechanism underlying immune stimulation by gp96.

Original languageEnglish
Pages (from-to)2042-2054
Number of pages13
JournalAmerican Journal of Pathology
Volume170
Issue number6
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Amino Acyl-tRNA Synthetases
Heat-Shock Proteins
Endoplasmic Reticulum
Autoimmune Diseases
Proteins
Dimerization
Ligases
Dendritic Cells
Carrier Proteins
Phenotype
KDEL receptor

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Han, Jung Min ; Sang, Gyu Park ; Liu, Bei ; Park, Bum Joon ; Jin, Young Kim ; Cheng, He Jin ; Yeong, Wook Song ; Li, Zihai ; Kim, Sunghoon. / Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96 : Its pathological implications in lupus-like autoimmune diseases. In: American Journal of Pathology. 2007 ; Vol. 170, No. 6. pp. 2042-2054.
@article{3cc8d91cf8e8419e8e7762bff13a5322,
title = "Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96: Its pathological implications in lupus-like autoimmune diseases",
abstract = "Aminoacyl-tRNA synthetase-interacting multifunctional protein 1 (AIMP1; previously known as p43) is a multifunctional protein that was initially found in multitRNA synthetase complex. In the present study, screening of the AIMP1-binding proteins revealed that AIMP1 can form a molecular complex with heat shock protein gp96. AIMP1 enhances gp96 dimerization and the interaction between gp96 and KDEL receptor-1 (KDELR-1), which mediates the retrieval of KDEL-containing proteins from Golgi to the endoplasmic reticulum (ER). The interaction between gp96 and KDELR-1 was reduced in AIMP1-deficient cells, and this disturbed ER retention of gp96 and increased its cell surface localization. Moreover, this localization of gp96 at the cell surface was suppressed by its interaction with AIMP1 and enhanced by the depletion of endogenous AIMP1. In addition, AIMP1-deficient mice showed dendritic cell activation attributable to increased gp96 surface presentation and lupus-like autoimmune phenotypes. These results suggest that AIMP1 acts as a regulator of the ER retention of gp96 and provide a new perspective of the regulatory mechanism underlying immune stimulation by gp96.",
author = "Han, {Jung Min} and Sang, {Gyu Park} and Bei Liu and Park, {Bum Joon} and Jin, {Young Kim} and Cheng, {He Jin} and Yeong, {Wook Song} and Zihai Li and Sunghoon Kim",
year = "2007",
month = "1",
day = "1",
doi = "10.2353/ajpath.2007.061266",
language = "English",
volume = "170",
pages = "2042--2054",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "6",

}

Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96 : Its pathological implications in lupus-like autoimmune diseases. / Han, Jung Min; Sang, Gyu Park; Liu, Bei; Park, Bum Joon; Jin, Young Kim; Cheng, He Jin; Yeong, Wook Song; Li, Zihai; Kim, Sunghoon.

In: American Journal of Pathology, Vol. 170, No. 6, 01.01.2007, p. 2042-2054.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96

T2 - Its pathological implications in lupus-like autoimmune diseases

AU - Han, Jung Min

AU - Sang, Gyu Park

AU - Liu, Bei

AU - Park, Bum Joon

AU - Jin, Young Kim

AU - Cheng, He Jin

AU - Yeong, Wook Song

AU - Li, Zihai

AU - Kim, Sunghoon

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Aminoacyl-tRNA synthetase-interacting multifunctional protein 1 (AIMP1; previously known as p43) is a multifunctional protein that was initially found in multitRNA synthetase complex. In the present study, screening of the AIMP1-binding proteins revealed that AIMP1 can form a molecular complex with heat shock protein gp96. AIMP1 enhances gp96 dimerization and the interaction between gp96 and KDEL receptor-1 (KDELR-1), which mediates the retrieval of KDEL-containing proteins from Golgi to the endoplasmic reticulum (ER). The interaction between gp96 and KDELR-1 was reduced in AIMP1-deficient cells, and this disturbed ER retention of gp96 and increased its cell surface localization. Moreover, this localization of gp96 at the cell surface was suppressed by its interaction with AIMP1 and enhanced by the depletion of endogenous AIMP1. In addition, AIMP1-deficient mice showed dendritic cell activation attributable to increased gp96 surface presentation and lupus-like autoimmune phenotypes. These results suggest that AIMP1 acts as a regulator of the ER retention of gp96 and provide a new perspective of the regulatory mechanism underlying immune stimulation by gp96.

AB - Aminoacyl-tRNA synthetase-interacting multifunctional protein 1 (AIMP1; previously known as p43) is a multifunctional protein that was initially found in multitRNA synthetase complex. In the present study, screening of the AIMP1-binding proteins revealed that AIMP1 can form a molecular complex with heat shock protein gp96. AIMP1 enhances gp96 dimerization and the interaction between gp96 and KDEL receptor-1 (KDELR-1), which mediates the retrieval of KDEL-containing proteins from Golgi to the endoplasmic reticulum (ER). The interaction between gp96 and KDELR-1 was reduced in AIMP1-deficient cells, and this disturbed ER retention of gp96 and increased its cell surface localization. Moreover, this localization of gp96 at the cell surface was suppressed by its interaction with AIMP1 and enhanced by the depletion of endogenous AIMP1. In addition, AIMP1-deficient mice showed dendritic cell activation attributable to increased gp96 surface presentation and lupus-like autoimmune phenotypes. These results suggest that AIMP1 acts as a regulator of the ER retention of gp96 and provide a new perspective of the regulatory mechanism underlying immune stimulation by gp96.

UR - http://www.scopus.com/inward/record.url?scp=34447295947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447295947&partnerID=8YFLogxK

U2 - 10.2353/ajpath.2007.061266

DO - 10.2353/ajpath.2007.061266

M3 - Article

C2 - 17525271

AN - SCOPUS:34447295947

VL - 170

SP - 2042

EP - 2054

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 6

ER -