Amperometric glucose biosensor based on glucose oxidase encapsulated in carbon nanotube-titania-Nafion composite film on platinized glassy carbon electrode

Nim Choi Han, Hoon Han Jee, Ae Park Ji, Min Lee Joong, Wonyong Lee

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A highly sensitive and selective glucose biosensor has been developed based on immobilization of glucose oxidase within mesoporous carbon nanotube-titania-Nafion composite film coated on a platinized glassy carbon electrode. Synergistic electrocatalytic activity of carbon nanotubes and electrodeposited platinum nanoparticles on electrode surface resulted in an efficient reduction of hydrogen peroxide, allowing the sensitive and selective quantitation of glucose by the direct reduction of enzymatically-liberated hydrogen peroxide at -0.1 V versus Ag/AgCl (3 M NaCl) without a mediator. The present biosensor responded linearly to glucose in the wide concentration range from 5.0 × 10-5 to 5.0 × 10-3 M with a good sensitivity of 154 mA M-1 cm-2. Due to the mesoporous nature of CNT-titania-Nafion composite film, the present biosensor exhibited very fast response time within 2 s. In addition, the present biosensor did not show any interference from large excess of ascorbic acid and uric acid.

Original languageEnglish
Pages (from-to)1757-1763
Number of pages7
JournalElectroanalysis
Volume19
Issue number17
DOIs
Publication statusPublished - 2007 Sep 1

Fingerprint

Glucose Oxidase
Carbon Nanotubes
Glucose oxidase
Glassy carbon
Composite films
Biosensors
Glucose
Carbon nanotubes
Titanium
Electrodes
Hydrogen peroxide
Hydrogen Peroxide
Ascorbic acid
Uric Acid
Platinum
Ascorbic Acid
Nanoparticles
Acids
titanium dioxide
perfluorosulfonic acid

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

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abstract = "A highly sensitive and selective glucose biosensor has been developed based on immobilization of glucose oxidase within mesoporous carbon nanotube-titania-Nafion composite film coated on a platinized glassy carbon electrode. Synergistic electrocatalytic activity of carbon nanotubes and electrodeposited platinum nanoparticles on electrode surface resulted in an efficient reduction of hydrogen peroxide, allowing the sensitive and selective quantitation of glucose by the direct reduction of enzymatically-liberated hydrogen peroxide at -0.1 V versus Ag/AgCl (3 M NaCl) without a mediator. The present biosensor responded linearly to glucose in the wide concentration range from 5.0 × 10-5 to 5.0 × 10-3 M with a good sensitivity of 154 mA M-1 cm-2. Due to the mesoporous nature of CNT-titania-Nafion composite film, the present biosensor exhibited very fast response time within 2 s. In addition, the present biosensor did not show any interference from large excess of ascorbic acid and uric acid.",
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Amperometric glucose biosensor based on glucose oxidase encapsulated in carbon nanotube-titania-Nafion composite film on platinized glassy carbon electrode. / Han, Nim Choi; Jee, Hoon Han; Ji, Ae Park; Joong, Min Lee; Lee, Wonyong.

In: Electroanalysis, Vol. 19, No. 17, 01.09.2007, p. 1757-1763.

Research output: Contribution to journalArticle

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