Amperometric glucose biosensor based on sol-gel-derived zirconia/Nafion composite film as encapsulation matrix

Hyun Jung Kim, Hyun Yoon Sook, Nim Choi Han, Young Ku Lyu, Won Yong Lee

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Abstract

An amperometric glucose biosensor has been developed based on the use of the nanoporous composite film of sol-gel-derived zirconia and perfluorosulfonated ionomer, Nafion, for the encapsulation of glucose oxidase (GOx) on a platinized glassy carbon electrode. Zirconium isopropoxide (ZrOPr) was used as a sol-gel precursor for the preparation of zirconia/Nafion composite film and the performance of the resulting glucose biosensor was tuned by controlling the water content in the acid-catalyzed hydrolysis of sol-gel stock solution. The presence of Nafion polymer in the sol-gel-derived zirconia in the biosensor resulted in faster response time and higher sensitivity compared to those obtained at the pure zirconia- and pure Nafion-based biosensors. Because of the nanoporous nature of the composite film, the glucose biosensor based on the zirconia/Nafion composite film can reach 95% of steady-state current less than 5 s. In addition, the biosensor responds to glucose linearly in the range of 0.03-15.08 mM with a sensitivity of 3.40 μA/mM and the detection limit of 0.037 mM (S/N = 3). Moreover, the biosensor exhibited good sensor-to-sensor reproducibility (∼5%) and long-term stability (90% of its original activity retained after 4 weeks) when stored in 50 mM phosphate buffer at pH 7 at 4°C.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume27
Issue number1
DOIs
Publication statusPublished - 2006 Jan 20

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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