An amperometric biosensor based on tyrosinase immobilized in silicate/Nafion composite film has been developed for the determination of phenolic compounds. The Nafion polymer in the composite was used not only to overcome the brittleness of the pure sol-gel-derived silicate film but also to increase the long-term stability of the biosensor. Tyrosinase was immobilized by a thin film of silicate/Nafion composite on a glassy carbon electrode. Phenolic compounds were determined by the direct reduction of biocatalytically-liberated quinone species at -200mV versus Ag/AgCl (3M NaCl). The process parameters for the fabrication of the enzyme electrode and various experimental variables such as pH and operating potential were explored for optimum analytical performance of the enzyme electrode. The biosensor can reach 95% of steady-state current in about 15s. The sensitivities of the biosensor for catechol and phenol were 200 and 46mA/M, respectively. A detection limit of 0.35mM catechol was obtained with a signal-to-noise ratio of 3. The enzyme electrode retained 74% of its initial activity after 2 weeks of storage in 50mM phosphate buffer at pH 7.
Bibliographical noteFunding Information:
This work was supported by Korea Research Foundation Grant (KRF-2000-015-DP0239).
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Environmental Chemistry