A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17nm) of the composite film, the biosensor exhibits very fast response time of 2s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7×10-8M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50mM phosphate buffer at pH7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry