Amperometric tyrosinase biosensor based on carbon nanotube-doped sol-gel-derived zinc oxide-Nafion composite films

Joong Min Lee, Guang Ri Xu, Byung Kun Kim, Han Nim Choi, Won Yong Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)


A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17nm) of the composite film, the biosensor exhibits very fast response time of 2s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7×10-8M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50mM phosphate buffer at pH7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.

Original languageEnglish
Pages (from-to)962-970
Number of pages9
Issue number4
Publication statusPublished - 2011 Apr 1


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry

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