An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis

Eun Joo Chung, Tae Yun Kim, Sung Jong Hong, Taisoon Yong

Research output: Contribution to journalArticle

Abstract

A cDNA clone encoding 8 kDa protein was retrieved from an EST pool of Chinese liver fluke Clonorchis sinensis. A deduced polypeptide of the cDNA clone was similar to 8 kDa Ca2+-binding proteins from other parasitic trematodes, and, thus, named as CsCa8, containing two EF-hand Ca 2+-binding sites. Homology models predicted CsCa8 to be a single globular structure having four helices and molecular folds similar to Ca 2+-binding state of other small Ca2+-binding proteins. Recombinant CsCa8 protein showed specific Ca2+-binding affinity and shifting in native gel mobility assay. Mouse immune sera raised against recombinant CsCa8 protein recognized native CsCa8 from adult C. sinensis worm extract. CsCa8 was localized in oral and ventral suckers, vitelline follicles and subtegumental tissues. These findings suggest that CsCa8 might be involved in cellular Ca2+ signal transduction for muscle contraction and egg production.

Original languageEnglish
Pages (from-to)4121-4128
Number of pages8
JournalParasitology Research
Volume112
Issue number12
DOIs
Publication statusPublished - 2013 Dec 1

Fingerprint

Clonorchis sinensis
liver flukes
Fasciola hepatica
Recombinant Proteins
binding proteins
Carrier Proteins
Complementary DNA
Clone Cells
EF Hand Motifs
calcium
Expressed Sequence Tags
Muscle Contraction
recombinant proteins
Ovum
Immune Sera
Signal Transduction
Gels
Binding Sites
clones
Peptides

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Chung, Eun Joo ; Kim, Tae Yun ; Hong, Sung Jong ; Yong, Taisoon. / An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis. In: Parasitology Research. 2013 ; Vol. 112, No. 12. pp. 4121-4128.
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An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis. / Chung, Eun Joo; Kim, Tae Yun; Hong, Sung Jong; Yong, Taisoon.

In: Parasitology Research, Vol. 112, No. 12, 01.12.2013, p. 4121-4128.

Research output: Contribution to journalArticle

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