An effective range of polydeoxyribonucleotides is critical for wound healing quality

Kyu Hee Hwang, Ji Hee Kim, Eun Young Park, Seungkuy Cha

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established. In the present study, the regeneration quality with low (<50 kDa), middle [classic PDRN; 50-1,500 kDa] and high (>1,500 kDa) molecular weight PDRNs in a skin wound healing mouse model was examined using hematoxylin and eosin, as well as Masson's trichrome stain. A 4 mm biopsy punch was used to produce wounds in the skin of the mice. PDRN-mediated cellular behavior and signaling were evaluated by in vitro scratch assay and western blot analysis, respectively. It was observed that the apparent surface wound healing processes were not significantly different between PDRN molecular sizes. Immunohistochemical analysis revealed that classic PDRN-injected mice exhibited less lipid accumulation with increased collagen composition. These results suggested that 50-1,500 kDa PDRN offers an effective DNA mixture to improve wound healing quality. Furthermore, classic PDRN increased cell migration via c-Jun N-terminal kinase signaling in human fibroblasts. The present study suggests an optimal PDRN molecular weight to promote wound healing, and novel approaches for therapeutic strategies to improve tissue regeneration quality.

Original languageEnglish
Pages (from-to)5166-5172
Number of pages7
JournalMolecular Medicine Reports
Volume18
Issue number6
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Polydeoxyribonucleotides
Wound Healing
Regeneration
Tissue regeneration
Skin
Oncorhynchus keta
Molecular Weight
Molecular weight
JNK Mitogen-Activated Protein Kinases
Biopsy
Oncorhynchus mykiss
DNA
Wounds and Injuries
Fibroblasts
Hematoxylin
Eosine Yellowish-(YS)
Cell Movement
Extracellular Matrix
Spermatozoa
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

Cite this

Hwang, Kyu Hee ; Kim, Ji Hee ; Park, Eun Young ; Cha, Seungkuy. / An effective range of polydeoxyribonucleotides is critical for wound healing quality. In: Molecular Medicine Reports. 2018 ; Vol. 18, No. 6. pp. 5166-5172.
@article{9b52b00fe3db4e5084a643c4d4ea1315,
title = "An effective range of polydeoxyribonucleotides is critical for wound healing quality",
abstract = "Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established. In the present study, the regeneration quality with low (<50 kDa), middle [classic PDRN; 50-1,500 kDa] and high (>1,500 kDa) molecular weight PDRNs in a skin wound healing mouse model was examined using hematoxylin and eosin, as well as Masson's trichrome stain. A 4 mm biopsy punch was used to produce wounds in the skin of the mice. PDRN-mediated cellular behavior and signaling were evaluated by in vitro scratch assay and western blot analysis, respectively. It was observed that the apparent surface wound healing processes were not significantly different between PDRN molecular sizes. Immunohistochemical analysis revealed that classic PDRN-injected mice exhibited less lipid accumulation with increased collagen composition. These results suggested that 50-1,500 kDa PDRN offers an effective DNA mixture to improve wound healing quality. Furthermore, classic PDRN increased cell migration via c-Jun N-terminal kinase signaling in human fibroblasts. The present study suggests an optimal PDRN molecular weight to promote wound healing, and novel approaches for therapeutic strategies to improve tissue regeneration quality.",
author = "Hwang, {Kyu Hee} and Kim, {Ji Hee} and Park, {Eun Young} and Seungkuy Cha",
year = "2018",
month = "12",
day = "1",
doi = "10.3892/mmr.2018.9539",
language = "English",
volume = "18",
pages = "5166--5172",
journal = "Molecular Medicine Reports",
issn = "1791-2997",
publisher = "Spandidos Publications",
number = "6",

}

An effective range of polydeoxyribonucleotides is critical for wound healing quality. / Hwang, Kyu Hee; Kim, Ji Hee; Park, Eun Young; Cha, Seungkuy.

In: Molecular Medicine Reports, Vol. 18, No. 6, 01.12.2018, p. 5166-5172.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An effective range of polydeoxyribonucleotides is critical for wound healing quality

AU - Hwang, Kyu Hee

AU - Kim, Ji Hee

AU - Park, Eun Young

AU - Cha, Seungkuy

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established. In the present study, the regeneration quality with low (<50 kDa), middle [classic PDRN; 50-1,500 kDa] and high (>1,500 kDa) molecular weight PDRNs in a skin wound healing mouse model was examined using hematoxylin and eosin, as well as Masson's trichrome stain. A 4 mm biopsy punch was used to produce wounds in the skin of the mice. PDRN-mediated cellular behavior and signaling were evaluated by in vitro scratch assay and western blot analysis, respectively. It was observed that the apparent surface wound healing processes were not significantly different between PDRN molecular sizes. Immunohistochemical analysis revealed that classic PDRN-injected mice exhibited less lipid accumulation with increased collagen composition. These results suggested that 50-1,500 kDa PDRN offers an effective DNA mixture to improve wound healing quality. Furthermore, classic PDRN increased cell migration via c-Jun N-terminal kinase signaling in human fibroblasts. The present study suggests an optimal PDRN molecular weight to promote wound healing, and novel approaches for therapeutic strategies to improve tissue regeneration quality.

AB - Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established. In the present study, the regeneration quality with low (<50 kDa), middle [classic PDRN; 50-1,500 kDa] and high (>1,500 kDa) molecular weight PDRNs in a skin wound healing mouse model was examined using hematoxylin and eosin, as well as Masson's trichrome stain. A 4 mm biopsy punch was used to produce wounds in the skin of the mice. PDRN-mediated cellular behavior and signaling were evaluated by in vitro scratch assay and western blot analysis, respectively. It was observed that the apparent surface wound healing processes were not significantly different between PDRN molecular sizes. Immunohistochemical analysis revealed that classic PDRN-injected mice exhibited less lipid accumulation with increased collagen composition. These results suggested that 50-1,500 kDa PDRN offers an effective DNA mixture to improve wound healing quality. Furthermore, classic PDRN increased cell migration via c-Jun N-terminal kinase signaling in human fibroblasts. The present study suggests an optimal PDRN molecular weight to promote wound healing, and novel approaches for therapeutic strategies to improve tissue regeneration quality.

UR - http://www.scopus.com/inward/record.url?scp=85056180663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056180663&partnerID=8YFLogxK

U2 - 10.3892/mmr.2018.9539

DO - 10.3892/mmr.2018.9539

M3 - Article

C2 - 30320361

AN - SCOPUS:85056180663

VL - 18

SP - 5166

EP - 5172

JO - Molecular Medicine Reports

JF - Molecular Medicine Reports

SN - 1791-2997

IS - 6

ER -