An experimental correlation study between field-target overlap and sensitivity of surface plasmon resonance biosensors based on sandwiched immunoassays

Yeonsoo Ryu, Seyoung Moon, Youngjin Oh, Yonghwi Kim, Donghyun Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this report, we have studied the effectiveness of field-target overlap to evaluate detection sensitivity of surface plasmon resonance (SPR) biosensors. The investigation used theoretical analysis based on the transfer matrix method, which was experimentally confirmed by thin film-based detection in sandwich and reverse sandwich immunoglobulin G (IgG) assays. Both theoretical and experimental results show that strong correlation exists between the overlap and the sensitivity with the coefficient of correlation higher than 95% in all the cases that we have considered. We have also confirmed the correlation in diffraction grating-based SPR measurement of IgG/anti-IgG interactions. The correlation elucidates the mechanism behind the far-field detection sensitivity of SPR biosensors and can lead to the enhancement of SPR biosensing with molecular scale sensitivity.

Original languageEnglish
Pages (from-to)4626-4631
Number of pages6
JournalOptics Communications
Volume285
Issue number21-22
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

immunoassay
Surface plasmon resonance
bioinstrumentation
surface plasmon resonance
Biosensors
Immunoglobulin G
Transfer matrix method
sensitivity
Diffraction gratings
gratings (spectra)
matrix methods
far fields
Assays
Thin films
augmentation
coefficients
thin films
interactions

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry
  • Electrical and Electronic Engineering

Cite this

@article{175062b2d04046a4a5ac3b701fce9d28,
title = "An experimental correlation study between field-target overlap and sensitivity of surface plasmon resonance biosensors based on sandwiched immunoassays",
abstract = "In this report, we have studied the effectiveness of field-target overlap to evaluate detection sensitivity of surface plasmon resonance (SPR) biosensors. The investigation used theoretical analysis based on the transfer matrix method, which was experimentally confirmed by thin film-based detection in sandwich and reverse sandwich immunoglobulin G (IgG) assays. Both theoretical and experimental results show that strong correlation exists between the overlap and the sensitivity with the coefficient of correlation higher than 95{\%} in all the cases that we have considered. We have also confirmed the correlation in diffraction grating-based SPR measurement of IgG/anti-IgG interactions. The correlation elucidates the mechanism behind the far-field detection sensitivity of SPR biosensors and can lead to the enhancement of SPR biosensing with molecular scale sensitivity.",
author = "Yeonsoo Ryu and Seyoung Moon and Youngjin Oh and Yonghwi Kim and Donghyun Kim",
year = "2012",
month = "10",
day = "1",
doi = "10.1016/j.optcom.2012.07.016",
language = "English",
volume = "285",
pages = "4626--4631",
journal = "Optics Communications",
issn = "0030-4018",
publisher = "Elsevier",
number = "21-22",

}

An experimental correlation study between field-target overlap and sensitivity of surface plasmon resonance biosensors based on sandwiched immunoassays. / Ryu, Yeonsoo; Moon, Seyoung; Oh, Youngjin; Kim, Yonghwi; Kim, Donghyun.

In: Optics Communications, Vol. 285, No. 21-22, 01.10.2012, p. 4626-4631.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An experimental correlation study between field-target overlap and sensitivity of surface plasmon resonance biosensors based on sandwiched immunoassays

AU - Ryu, Yeonsoo

AU - Moon, Seyoung

AU - Oh, Youngjin

AU - Kim, Yonghwi

AU - Kim, Donghyun

PY - 2012/10/1

Y1 - 2012/10/1

N2 - In this report, we have studied the effectiveness of field-target overlap to evaluate detection sensitivity of surface plasmon resonance (SPR) biosensors. The investigation used theoretical analysis based on the transfer matrix method, which was experimentally confirmed by thin film-based detection in sandwich and reverse sandwich immunoglobulin G (IgG) assays. Both theoretical and experimental results show that strong correlation exists between the overlap and the sensitivity with the coefficient of correlation higher than 95% in all the cases that we have considered. We have also confirmed the correlation in diffraction grating-based SPR measurement of IgG/anti-IgG interactions. The correlation elucidates the mechanism behind the far-field detection sensitivity of SPR biosensors and can lead to the enhancement of SPR biosensing with molecular scale sensitivity.

AB - In this report, we have studied the effectiveness of field-target overlap to evaluate detection sensitivity of surface plasmon resonance (SPR) biosensors. The investigation used theoretical analysis based on the transfer matrix method, which was experimentally confirmed by thin film-based detection in sandwich and reverse sandwich immunoglobulin G (IgG) assays. Both theoretical and experimental results show that strong correlation exists between the overlap and the sensitivity with the coefficient of correlation higher than 95% in all the cases that we have considered. We have also confirmed the correlation in diffraction grating-based SPR measurement of IgG/anti-IgG interactions. The correlation elucidates the mechanism behind the far-field detection sensitivity of SPR biosensors and can lead to the enhancement of SPR biosensing with molecular scale sensitivity.

UR - http://www.scopus.com/inward/record.url?scp=84865676589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865676589&partnerID=8YFLogxK

U2 - 10.1016/j.optcom.2012.07.016

DO - 10.1016/j.optcom.2012.07.016

M3 - Article

VL - 285

SP - 4626

EP - 4631

JO - Optics Communications

JF - Optics Communications

SN - 0030-4018

IS - 21-22

ER -