An experimental correlation study between field-target overlap and sensitivity of surface plasmon resonance biosensors based on sandwiched immunoassays

Yeonsoo Ryu, Seyoung Moon, Youngjin Oh, Yonghwi Kim, Donghyun Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this report, we have studied the effectiveness of field-target overlap to evaluate detection sensitivity of surface plasmon resonance (SPR) biosensors. The investigation used theoretical analysis based on the transfer matrix method, which was experimentally confirmed by thin film-based detection in sandwich and reverse sandwich immunoglobulin G (IgG) assays. Both theoretical and experimental results show that strong correlation exists between the overlap and the sensitivity with the coefficient of correlation higher than 95% in all the cases that we have considered. We have also confirmed the correlation in diffraction grating-based SPR measurement of IgG/anti-IgG interactions. The correlation elucidates the mechanism behind the far-field detection sensitivity of SPR biosensors and can lead to the enhancement of SPR biosensing with molecular scale sensitivity.

Original languageEnglish
Pages (from-to)4626-4631
Number of pages6
JournalOptics Communications
Volume285
Issue number21-22
DOIs
Publication statusPublished - 2012 Oct 1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry
  • Electrical and Electronic Engineering

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