The cellular localization and spatiotemporal expression pattern of APG-2 protein, a member of the heat shock protein 110 family, were investigated in the rat hippocampus after transient forebrain ischemia. The spatiotemporal patterns of immunoreactivity of both APG-2 and glial fibrillary acidic protein were very similar, indicating that reactive astrocytes express APG-2, which was confirmed by double immunofluorescence histochemistry. Colocalization of APG-2 and a neuronal marker NeuN in the neurons of the CA2 and CA3 subfields was also confirmed.
Bibliographical noteFunding Information:
The authors thank Mrs. Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance. This research was supported by The Catholic Medical Center Research Foundation established in 2000 (to M.-Y.L. and S.Y.K).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Clinical Neurology
- Developmental Biology