An implantable neural probe with monolithically integrated dielectric waveguide and recording electrodes for optogenetics applications

Fan Wu, Eran Stark, Maesoon Im, Il Joo Cho, Eui Sung Yoon, György Buzsáki, Kensall D. Wise, Euisik Yoon

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Objective. Optogenetics promises exciting neuroscience research by offering optical stimulation of neurons with unprecedented temporal resolution, cell-type specificity and the ability to excite as well as to silence neurons. This work provides the technical solution to deliver light to local neurons and record neural potentials, facilitating local circuit analysis and bridging the gap between optogenetics and neurophysiology research. Approach. We have designed and obtained the first in vivo validation of a neural probe with monolithically integrated electrodes and waveguide. High spatial precision enables optical excitation of targeted neurons with minimal power and recording of single-units in dense cortical and subcortical regions. Main results. The total coupling and transmission loss through the dielectric waveguide at 473 nm was 10.5 ± 1.9 dB, corresponding to an average output intensity of 9400 mW mm-2 when coupled to a 7 mW optical fiber. Spontaneous field potentials and spiking activities of multiple Channelrhodopsin-2 expressing neurons were recorded in the hippocampus CA1 region of an anesthetized rat. Blue light stimulation at intensity of 51 mW mm-2 induced robust spiking activities in the physiologically identified local populations. Significance. This minimally invasive, complete monolithic integration provides unmatched spatial precision and scalability for future optogenetics studies at deep brain regions with high neuronal density.

Original languageEnglish
Article number056012
JournalJournal of Neural Engineering
Volume10
Issue number5
DOIs
Publication statusPublished - 2013 Oct 1

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Optogenetics
Dielectric waveguides
Neurons
Electrodes
Neurophysiology
Optical Fibers
Light
Photoexcitation
Neurosciences
Electric network analysis
Research
Scalability
Rats
Optical fibers
Hippocampus
Brain
Waveguides
Population

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Wu, Fan ; Stark, Eran ; Im, Maesoon ; Cho, Il Joo ; Yoon, Eui Sung ; Buzsáki, György ; Wise, Kensall D. ; Yoon, Euisik. / An implantable neural probe with monolithically integrated dielectric waveguide and recording electrodes for optogenetics applications. In: Journal of Neural Engineering. 2013 ; Vol. 10, No. 5.
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An implantable neural probe with monolithically integrated dielectric waveguide and recording electrodes for optogenetics applications. / Wu, Fan; Stark, Eran; Im, Maesoon; Cho, Il Joo; Yoon, Eui Sung; Buzsáki, György; Wise, Kensall D.; Yoon, Euisik.

In: Journal of Neural Engineering, Vol. 10, No. 5, 056012, 01.10.2013.

Research output: Contribution to journalArticle

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