This article presents a thermal biosensor to diagnose the anemia without chemical treatments using temperature increase of red blood cells (RBC) when hemoglobin molecules absorb specific wavelength of photons and convert them to thermal energy. For measuring temperature change of red blood cell, the micro-scaled platinum resistance temperature detector (Pt RTD) was developed. For maintenance of constant ambient temperature, we designed and fabricated a thermostat system. The thermostat system consists of a K-type thermocouple and two electric heaters that serve to increase the system temperature, which is monitored by the thermocouple. Both heaters and the thermocouple were connected to a proportional-integral-derivative (PID) controller and enabled to maintain the temperature constant (<±0.1°C). For specific heating of red blood cell, 8.0W/cm2 diode pumped solid state (DPSS) continuous wave (CW) laser module was used with 532nm wavelength. Using this system, we successfully measured the temperature variations (from 66.33±2.72°C to 74.16±2.06°C) of whole blood samples from 10 anemic patients and subsequently determined the concentration of hemoglobin (from 7.2g/dL to 9.8g/dL). The method proposed in this paper requires significantly less amount of whole blood sample (6μl) compared with the conventional methods (175μl) and allows instantaneous diagnosis (3s) of anemia.
Bibliographical noteFunding Information:
This work was supported by the “System IC 2010” project of the Korea Ministry of Knowledge Economy (10030554-2008-02), and the MOCIE program (a sabbatical grant Y2009 for promoting technological innovation in industry). The facilities were provided by KOSEF through the National Core Research Center for Nanomedical Technology (R15-2004-024-00000-0) and ICBIN of Seoul R&BD program (Grant no. 10816).
All Science Journal Classification (ASJC) codes
- Biomedical Engineering