An integrated systems biology approach identifies positive cofactor 4 as a factor that increases reprogramming efficiency

Junghyun Jo, Sohyun Hwang, Hyung Joon Kim, Soomin Hong, Jeoung Eun Lee, Sung Geum Lee, Ahmi Baek, Heonjong Han, Jin Il Lee, Insuk Lee, Dong Ryul Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Spermatogonial stem cells (SSCs) can spontaneously dedifferentiate into embryonic stem cell (ESC)-like cells, which are designated as multipotent SSCs (mSSCs), without ectopic expression of reprogramming factors. Interestingly, SSCs express key pluripotency genes such asOct4,Sox2, Klf4 andMyc. Therefore, molecular dissection of mSSC reprogramming may provide clues about novel endogenous reprogramming or pluripotency regulatory factors. Our comparative transcriptome analysis of mSSCs and induced pluripotent stem cells (iPSCs) suggests that they have similar pluripotency states but are reprogrammed via different transcriptional pathways. We identified 53 genes as putative pluripotency regulatory factors using an integrated systems biology approach. We demonstrated a selected candidate, Positive cofactor 4 (Pc4), can enhance the efficiency of somatic cell reprogramming by promoting and maintaining transcriptional activity of the key reprograming factors. These results suggest that Pc4 has an important role in inducing spontaneous somatic cell reprogramming via up-regulation of key pluripotency genes.

Original languageEnglish
Pages (from-to)1203-1215
Number of pages13
JournalNucleic acids research
Volume44
Issue number3
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Genetics

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    Jo, J., Hwang, S., Kim, H. J., Hong, S., Lee, J. E., Lee, S. G., Baek, A., Han, H., Lee, J. I., Lee, I., & Lee, D. R. (2016). An integrated systems biology approach identifies positive cofactor 4 as a factor that increases reprogramming efficiency. Nucleic acids research, 44(3), 1203-1215. https://doi.org/10.1093/nar/gkv1468