An isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections

Bonhan Koo, Choong Eun Jin, Tae Yoon Lee, Jeong Hoon Lee, Mi Kyoung Park, Heungsup Sung, Se Yoon Park, Hyun Jung Lee, Sun Mi Kim, Ji Yeun Kim, Sung Han Kim, Yong Shin

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Recently, RNA viral infections caused by respiratory viruses, such as influenza, parainfluenza, respiratory syncytial virus, coronavirus, and Middle East respiratory syndrome-coronavirus (MERS-CoV), and Zika virus, are a major public health threats in the world. Although myriads of diagnostic methods based on RNA amplification have been developed in the last decades, they continue to lack speed, sensitivity, and specificity for clinical use. A rapid and accurate diagnostic method is needed for appropriate control, including isolation and treatment of the patients. Here, we report an isothermal, label-free, one-step RNA amplification and detection system, termed as iROAD, for the diagnosis of respiratory diseases. It couples a one-step isothermal RNA amplification method and a bio-optical sensor for simultaneous viral RNA amplification/detection in a label-free and real-time manner. The iROAD assay offers a one-step viral RNA amplification/detection example to rapid analysis (<20 min). The detection limit of iROAD assay was found to be 10-times more sensitive than that of real-time reverse transcription-PCR method. We confirmed the clinical utility of the iROAD assay by detecting viral RNAs obtained from 63 human respiratory samples. We envision that the iROAD assay will be useful and potentially adaptable for better diagnosis of emerging infectious diseases including respiratory diseases.

Original languageEnglish
Pages (from-to)187-194
Number of pages8
JournalBiosensors and Bioelectronics
Volume90
DOIs
Publication statusPublished - 2017 Apr 15

Bibliographical note

Funding Information:
This work was supported by the grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare , Republic of Korea ( HI15C-2774-020015 ), and also supported by a grant ( W16-701 ) from the Asan Institute for Life Sciences, Asan Medical Center , Seoul, Republic of Korea.

Publisher Copyright:
© 2016 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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