A new 7-nitrobenz-2-oxa-1,3-diazole (NBD) based colorimetric and fluorescence chemosensor for Zn2+, an ion involved in many biological processes, was designed and synthesized. The NBD-probe 1 displays a red-to-yellow color change and an enhancement of fluorescent intensity in the presence of an aqueous solution of Zn2+ ions (pH 7.2). Internal charge transfer (ICT) and photoinduced electron transfer (PET) mechanisms are responsible for these changes. The practical use of this probe was demonstrated by its application to the biologically relevant detection of Zn2+ ions in pancreatic β-cells.
Bibliographical noteFunding Information:
This work was supported by grants from the NRL program (R04-2007-000-2007-0 and R0A-2005-000-10027-0), the SRC program of KOSEF/MEST (R11-2005-008 and R11-2000-070), and WCU (R31-2008-000-10010-0) program. G.-H.K and M.J.C. also thank the BK21 program (KRF). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.
All Science Journal Classification (ASJC) codes
- Drug Discovery
- Organic Chemistry