An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection

Dongkyu Kang; Hyun Joo Ahn; Jiho Lee; Sang Kyung Kim; Jaechul Pyun; Chang Seon Song; Sang Jick Kim; Joonseok Lee

doi:10.1016/j.bios.2021.113369

An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection

Dongkyu Kang, Hyun Joo Ahn, Jiho Lee, Sang Kyung Kim, Jaechul Pyun, Chang Seon Song, Sang Jick Kim, Joonseok Lee

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.

Original language	English
Article number	113369
Journal	Biosensors and Bioelectronics
Volume	190
DOIs	https://doi.org/10.1016/j.bios.2021.113369
Publication status	Published - 2021 Oct 15

Bibliographical note

Funding Information:
This work was supported by the R&D Convergence Program of National Research Council of Science & Technology [grant number CAP-16-02-KIST/CRC-20-02-KIST ], Korea Institute of Science and Technology Institutional Program [grant number 2E3115A/2Z06270-20-137 ], KRIBB Research Initiative Program , and National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) [grant number 2020R1A2C210111111 ].

Publisher Copyright:
© 2021 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2021.113369

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@article{ffbd4b0c8dc941f69e094c24e65f9285,

title = "An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection",

abstract = "Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.",

author = "Dongkyu Kang and Ahn, {Hyun Joo} and Jiho Lee and Kim, {Sang Kyung} and Jaechul Pyun and Song, {Chang Seon} and Kim, {Sang Jick} and Joonseok Lee",

note = "Funding Information: This work was supported by the R&D Convergence Program of National Research Council of Science & Technology [grant number CAP-16-02-KIST/CRC-20-02-KIST ], Korea Institute of Science and Technology Institutional Program [grant number 2E3115A/2Z06270-20-137 ], KRIBB Research Initiative Program , and National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) [grant number 2020R1A2C210111111 ]. Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

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doi = "10.1016/j.bios.2021.113369",

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An NIR dual-emitting/absorbing inorganic compact pair : A self-calibrating LRET system for homogeneous virus detection. / Kang, Dongkyu; Ahn, Hyun Joo; Lee, Jiho; Kim, Sang Kyung; Pyun, Jaechul; Song, Chang Seon; Kim, Sang Jick; Lee, Joonseok.

In: Biosensors and Bioelectronics, Vol. 190, 113369, 15.10.2021.

Research output: Contribution to journal › Article › peer-review

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AU - Pyun, Jaechul

AU - Song, Chang Seon

AU - Kim, Sang Jick

AU - Lee, Joonseok

N1 - Funding Information: This work was supported by the R&D Convergence Program of National Research Council of Science & Technology [grant number CAP-16-02-KIST/CRC-20-02-KIST ], Korea Institute of Science and Technology Institutional Program [grant number 2E3115A/2Z06270-20-137 ], KRIBB Research Initiative Program , and National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) [grant number 2020R1A2C210111111 ]. Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/10/15

Y1 - 2021/10/15

N2 - Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.

AB - Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.

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An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection

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