An NMR approach to structural proteomics

Adelinda Yee, Xiaoqing Chang, Antonio Pineda-Lucena, Bin Wu, Anthony Semesi, Brian Le, Theresa Ramelot, Gregory M. Lee, Sudeepa Bhattacharyya, Pablo Gutierrez, Aleksej Denisov, Chang Hun Lee, John R. Cort, Guennadi Kozlov, Jack Liao, Grzegorz Finak, Limin Chen, David Wishart, Weontae Lee, Lawrence P. McIntoshKalle Gehring, Michael A. Kennedy, Aled M. Edwards, Cheryl H. Arrowsmith

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Abstract

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

Original languageEnglish
Pages (from-to)1825-1830
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number4
DOIs
Publication statusPublished - 2002 Feb 19

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All Science Journal Classification (ASJC) codes

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Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G. M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C. H., Cort, J. R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., ... Arrowsmith, C. H. (2002). An NMR approach to structural proteomics. Proceedings of the National Academy of Sciences of the United States of America, 99(4), 1825-1830. https://doi.org/10.1073/pnas.042684599