An NMR approach to structural proteomics

Adelinda Yee, Xiaoqing Chang, Antonio Pineda-Lucena, Bin Wu, Anthony Semesi, Brian Le, Theresa Ramelot, Gregory M. Lee, Sudeepa Bhattacharyya, Pablo Gutierrez, Aleksej Denisov, Chang Hun Lee, John R. Cort, Guennadi Kozlov, Jack Liao, Grzegorz Finak, Limin Chen, David Wishart, Weon Tae Lee, Lawrence P. McIntosh & 4 others Kalle Gehring, Michael A. Kennedy, Aled M. Edwards, Cheryl H. Arrowsmith

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

Original languageEnglish
Pages (from-to)1825-1830
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number4
DOIs
Publication statusPublished - 2002 Feb 19

Fingerprint

Proteomics
Proteins
Biomolecular Nuclear Magnetic Resonance
Proteome
Genome

All Science Journal Classification (ASJC) codes

  • General

Cite this

Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., ... Arrowsmith, C. H. (2002). An NMR approach to structural proteomics. Proceedings of the National Academy of Sciences of the United States of America, 99(4), 1825-1830. https://doi.org/10.1073/pnas.042684599
Yee, Adelinda ; Chang, Xiaoqing ; Pineda-Lucena, Antonio ; Wu, Bin ; Semesi, Anthony ; Le, Brian ; Ramelot, Theresa ; Lee, Gregory M. ; Bhattacharyya, Sudeepa ; Gutierrez, Pablo ; Denisov, Aleksej ; Lee, Chang Hun ; Cort, John R. ; Kozlov, Guennadi ; Liao, Jack ; Finak, Grzegorz ; Chen, Limin ; Wishart, David ; Lee, Weon Tae ; McIntosh, Lawrence P. ; Gehring, Kalle ; Kennedy, Michael A. ; Edwards, Aled M. ; Arrowsmith, Cheryl H. / An NMR approach to structural proteomics. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 4. pp. 1825-1830.
@article{7f3c5326836b4247885a151b38764d28,
title = "An NMR approach to structural proteomics",
abstract = "The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20{\%} of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.",
author = "Adelinda Yee and Xiaoqing Chang and Antonio Pineda-Lucena and Bin Wu and Anthony Semesi and Brian Le and Theresa Ramelot and Lee, {Gregory M.} and Sudeepa Bhattacharyya and Pablo Gutierrez and Aleksej Denisov and Lee, {Chang Hun} and Cort, {John R.} and Guennadi Kozlov and Jack Liao and Grzegorz Finak and Limin Chen and David Wishart and Lee, {Weon Tae} and McIntosh, {Lawrence P.} and Kalle Gehring and Kennedy, {Michael A.} and Edwards, {Aled M.} and Arrowsmith, {Cheryl H.}",
year = "2002",
month = "2",
day = "19",
doi = "10.1073/pnas.042684599",
language = "English",
volume = "99",
pages = "1825--1830",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "4",

}

Yee, A, Chang, X, Pineda-Lucena, A, Wu, B, Semesi, A, Le, B, Ramelot, T, Lee, GM, Bhattacharyya, S, Gutierrez, P, Denisov, A, Lee, CH, Cort, JR, Kozlov, G, Liao, J, Finak, G, Chen, L, Wishart, D, Lee, WT, McIntosh, LP, Gehring, K, Kennedy, MA, Edwards, AM & Arrowsmith, CH 2002, 'An NMR approach to structural proteomics', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 4, pp. 1825-1830. https://doi.org/10.1073/pnas.042684599

An NMR approach to structural proteomics. / Yee, Adelinda; Chang, Xiaoqing; Pineda-Lucena, Antonio; Wu, Bin; Semesi, Anthony; Le, Brian; Ramelot, Theresa; Lee, Gregory M.; Bhattacharyya, Sudeepa; Gutierrez, Pablo; Denisov, Aleksej; Lee, Chang Hun; Cort, John R.; Kozlov, Guennadi; Liao, Jack; Finak, Grzegorz; Chen, Limin; Wishart, David; Lee, Weon Tae; McIntosh, Lawrence P.; Gehring, Kalle; Kennedy, Michael A.; Edwards, Aled M.; Arrowsmith, Cheryl H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 4, 19.02.2002, p. 1825-1830.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An NMR approach to structural proteomics

AU - Yee, Adelinda

AU - Chang, Xiaoqing

AU - Pineda-Lucena, Antonio

AU - Wu, Bin

AU - Semesi, Anthony

AU - Le, Brian

AU - Ramelot, Theresa

AU - Lee, Gregory M.

AU - Bhattacharyya, Sudeepa

AU - Gutierrez, Pablo

AU - Denisov, Aleksej

AU - Lee, Chang Hun

AU - Cort, John R.

AU - Kozlov, Guennadi

AU - Liao, Jack

AU - Finak, Grzegorz

AU - Chen, Limin

AU - Wishart, David

AU - Lee, Weon Tae

AU - McIntosh, Lawrence P.

AU - Gehring, Kalle

AU - Kennedy, Michael A.

AU - Edwards, Aled M.

AU - Arrowsmith, Cheryl H.

PY - 2002/2/19

Y1 - 2002/2/19

N2 - The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

AB - The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

UR - http://www.scopus.com/inward/record.url?scp=18244366113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18244366113&partnerID=8YFLogxK

U2 - 10.1073/pnas.042684599

DO - 10.1073/pnas.042684599

M3 - Article

VL - 99

SP - 1825

EP - 1830

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 4

ER -