An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma

Shaheed Ur Rehman, Min Sun Choi, In Sook Kim, Seung Hyun Kim, Hye Hyun Yoo

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3 Citations (Scopus)

Abstract

Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). For sample preparation, plasma proteins were precipitated with 0.1% acetic acid in acetonitrile. Waters UPLC BEH C18 (2.1 mm I.D. × 100 mm, 1.7 μm) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02% acetic acid in distilled water (solvent A) and 0.02% acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3 mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7 → m/z 469.2 for hederacoside C and m/z 1108.3 → m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10–1000 ng/mL. The intra- and inter-day precision and accuracy were within 5%. The developed UPLC–MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance.

Original languageEnglish
Pages (from-to)90-95
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume129
DOIs
Publication statusPublished - 2016 Sep 10

Fingerprint

High performance liquid chromatography
Rats
High Pressure Liquid Chromatography
Plasmas
Hedera
Acetic Acid
Pharmaceutical Preparations
Pulmonary diseases
Pharmacokinetics
Water
Monitoring
Traditional Medicine
Tandem Mass Spectrometry
Calibration
Biological Availability
Medicine
Ionization
Mass spectrometry
Gastrointestinal Tract
hederacoside C

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

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title = "An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma",
abstract = "Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). For sample preparation, plasma proteins were precipitated with 0.1{\%} acetic acid in acetonitrile. Waters UPLC BEH C18 (2.1 mm I.D. × 100 mm, 1.7 μm) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02{\%} acetic acid in distilled water (solvent A) and 0.02{\%} acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3 mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7 → m/z 469.2 for hederacoside C and m/z 1108.3 → m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10–1000 ng/mL. The intra- and inter-day precision and accuracy were within 5{\%}. The developed UPLC–MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance.",
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AU - Choi, Min Sun

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AU - Kim, Seung Hyun

AU - Yoo, Hye Hyun

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