Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis

Yanlei Liu, Kan Lam, Yajarayma J. Tang, Paul H. Gumerlock, DongKi Lee, Myung Hwa Kim, Sum P. Lee, Joseph Silva, Joseph W. Leung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.

Original languageEnglish
Pages (from-to)858-861
Number of pages4
JournalChinese Medical Journal
Volume113
Issue number9
Publication statusPublished - 2000 Sep 1

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Bacteroides fragilis
Anaerobic Bacteria
Clostridium
rRNA Genes
Polymerase Chain Reaction
Glutamate-Ammonia Ligase
Molecular Biology
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Liu, Y., Lam, K., Tang, Y. J., Gumerlock, P. H., Lee, D., Kim, M. H., ... Leung, J. W. (2000). Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis. Chinese Medical Journal, 113(9), 858-861.
Liu, Yanlei ; Lam, Kan ; Tang, Yajarayma J. ; Gumerlock, Paul H. ; Lee, DongKi ; Kim, Myung Hwa ; Lee, Sum P. ; Silva, Joseph ; Leung, Joseph W. / Anaerobic bacteria and intrahepatic stones : Detections of Clostridium sp. and Bacteroides fragilis. In: Chinese Medical Journal. 2000 ; Vol. 113, No. 9. pp. 858-861.
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abstract = "Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83{\%}, 49/59). The two most common groups were detected in 19 (41{\%}) and 17 (37{\%}) of the 46 samples using SSPC analysis, and 25/59 (42{\%}) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.",
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Liu, Y, Lam, K, Tang, YJ, Gumerlock, PH, Lee, D, Kim, MH, Lee, SP, Silva, J & Leung, JW 2000, 'Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis', Chinese Medical Journal, vol. 113, no. 9, pp. 858-861.

Anaerobic bacteria and intrahepatic stones : Detections of Clostridium sp. and Bacteroides fragilis. / Liu, Yanlei; Lam, Kan; Tang, Yajarayma J.; Gumerlock, Paul H.; Lee, DongKi; Kim, Myung Hwa; Lee, Sum P.; Silva, Joseph; Leung, Joseph W.

In: Chinese Medical Journal, Vol. 113, No. 9, 01.09.2000, p. 858-861.

Research output: Contribution to journalArticle

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T1 - Anaerobic bacteria and intrahepatic stones

T2 - Detections of Clostridium sp. and Bacteroides fragilis

AU - Liu, Yanlei

AU - Lam, Kan

AU - Tang, Yajarayma J.

AU - Gumerlock, Paul H.

AU - Lee, DongKi

AU - Kim, Myung Hwa

AU - Lee, Sum P.

AU - Silva, Joseph

AU - Leung, Joseph W.

PY - 2000/9/1

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N2 - Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.

AB - Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.

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