Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.
| Original language | English |
|---|---|
| Title of host publication | Single Molecule Spectroscopy and Superresolution Imaging XII |
| Editors | Felix Koberling, Zygmunt K. Gryczynski, Ingo Gregor |
| Publisher | SPIE |
| ISBN (Electronic) | 9781510624108 |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
| Event | Single Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States Duration: 2019 Feb 2 → 2019 Feb 3 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 10884 |
| ISSN (Print) | 1605-7422 |
Conference
| Conference | Single Molecule Spectroscopy and Superresolution Imaging XII 2019 |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 19/2/2 → 19/2/3 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
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Analog mean-delay method : A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. / Kim, Dug Young; Hwang, Wonsang; Kim, Dong Eun; Won, Youngjae; Moon, Sucbei; Lee, Sang Yoon; Kang, Min Gu; Han, Won Sub.
Single Molecule Spectroscopy and Superresolution Imaging XII. ed. / Felix Koberling; Zygmunt K. Gryczynski; Ingo Gregor. SPIE, 2019. 108840D (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Analog mean-delay method
T2 - A new time-domain super-resolution technique for accurate fluorescence lifetime measurement
AU - Kim, Dug Young
AU - Hwang, Wonsang
AU - Kim, Dong Eun
AU - Won, Youngjae
AU - Moon, Sucbei
AU - Lee, Sang Yoon
AU - Kang, Min Gu
AU - Han, Won Sub
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.
AB - Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.
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UR - http://www.scopus.com/inward/citedby.url?scp=85066747278&partnerID=8YFLogxK
U2 - 10.1117/12.2510937
DO - 10.1117/12.2510937
M3 - Conference contribution
AN - SCOPUS:85066747278
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Single Molecule Spectroscopy and Superresolution Imaging XII
A2 - Koberling, Felix
A2 - Gryczynski, Zygmunt K.
A2 - Gregor, Ingo
PB - SPIE
ER -