Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement

Dug Young Kim, Wonsang Hwang, Dong Eun Kim, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Min Gu Kang, Won Sub Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging XII
EditorsFelix Koberling, Zygmunt K. Gryczynski, Ingo Gregor
PublisherSPIE
ISBN (Electronic)9781510624108
DOIs
Publication statusPublished - 2019 Jan 1
EventSingle Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States
Duration: 2019 Feb 22019 Feb 3

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10884
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging XII 2019
CountryUnited States
CitySan Francisco
Period19/2/219/2/3

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, D. Y., Hwang, W., Kim, D. E., Won, Y., Moon, S., Lee, S. Y., Kang, M. G., & Han, W. S. (2019). Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. In F. Koberling, Z. K. Gryczynski, & I. Gregor (Eds.), Single Molecule Spectroscopy and Superresolution Imaging XII [108840D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884). SPIE. https://doi.org/10.1117/12.2510937