Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.
Original language | English |
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Title of host publication | Single Molecule Spectroscopy and Superresolution Imaging XII |
Editors | Felix Koberling, Zygmunt K. Gryczynski, Ingo Gregor |
Publisher | SPIE |
ISBN (Electronic) | 9781510624108 |
DOIs | |
Publication status | Published - 2019 |
Event | Single Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States Duration: 2019 Feb 2 → 2019 Feb 3 |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 10884 |
ISSN (Print) | 1605-7422 |
Conference
Conference | Single Molecule Spectroscopy and Superresolution Imaging XII 2019 |
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Country/Territory | United States |
City | San Francisco |
Period | 19/2/2 → 19/2/3 |
Bibliographical note
Publisher Copyright:© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging