Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement

Dug Young Kim; Wonsang Hwang; Dong Eun Kim; Youngjae Won; Sucbei Moon; Sang Yoon Lee; Min Gu Kang; Won Sub Han

doi:10.1117/12.2510937

Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement

Dug Young Kim, Wonsang Hwang, Dong Eun Kim, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Min Gu Kang, Won Sub Han

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.

Original language	English
Title of host publication	Single Molecule Spectroscopy and Superresolution Imaging XII
Editors	Felix Koberling, Zygmunt K. Gryczynski, Ingo Gregor
Publisher	SPIE
ISBN (Electronic)	9781510624108
DOIs	https://doi.org/10.1117/12.2510937
Publication status	Published - 2019 Jan 1
Event	Single Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States Duration: 2019 Feb 2 → 2019 Feb 3

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10884
ISSN (Print)	1605-7422

Conference

Conference	Single Molecule Spectroscopy and Superresolution Imaging XII 2019
Country	United States
City	San Francisco
Period	19/2/2 → 19/2/3

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All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Cite this

Kim, D. Y., Hwang, W., Kim, D. E., Won, Y., Moon, S., Lee, S. Y., Kang, M. G., & Han, W. S. (2019). Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. In F. Koberling, Z. K. Gryczynski, & I. Gregor (Eds.), Single Molecule Spectroscopy and Superresolution Imaging XII [108840D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884). SPIE. https://doi.org/10.1117/12.2510937

Kim, Dug Young ; Hwang, Wonsang ; Kim, Dong Eun ; Won, Youngjae ; Moon, Sucbei ; Lee, Sang Yoon ; Kang, Min Gu ; Han, Won Sub. / Analog mean-delay method : A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. Single Molecule Spectroscopy and Superresolution Imaging XII. editor / Felix Koberling ; Zygmunt K. Gryczynski ; Ingo Gregor. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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abstract = "Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.",

author = "Kim, {Dug Young} and Wonsang Hwang and Kim, {Dong Eun} and Youngjae Won and Sucbei Moon and Lee, {Sang Yoon} and Kang, {Min Gu} and Han, {Won Sub}",

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Kim, DY, Hwang, W, Kim, DE, Won, Y, Moon, S, Lee, SY, Kang, MG & Han, WS 2019, Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. in F Koberling, ZK Gryczynski & I Gregor (eds), Single Molecule Spectroscopy and Superresolution Imaging XII., 108840D, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10884, SPIE, Single Molecule Spectroscopy and Superresolution Imaging XII 2019, San Francisco, United States, 19/2/2. https://doi.org/10.1117/12.2510937

Analog mean-delay method : A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. / Kim, Dug Young; Hwang, Wonsang; Kim, Dong Eun; Won, Youngjae; Moon, Sucbei; Lee, Sang Yoon; Kang, Min Gu; Han, Won Sub.

Single Molecule Spectroscopy and Superresolution Imaging XII. ed. / Felix Koberling; Zygmunt K. Gryczynski; Ingo Gregor. SPIE, 2019. 108840D (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.

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Kim DY, Hwang W, Kim DE, Won Y, Moon S, Lee SY et al. Analog mean-delay method: A new time-domain super-resolution technique for accurate fluorescence lifetime measurement. In Koberling F, Gryczynski ZK, Gregor I, editors, Single Molecule Spectroscopy and Superresolution Imaging XII. SPIE. 2019. 108840D. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2510937

Access to Document

10.1117/12.2510937