Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

Dong Uk Kim, Jacqueline Hayles, Dongsup Kim, Valerie Wood, Han Oh Park, Misun Won, Hyang Sook Yoo, Trevor Duhig, Miyoung Nam, Georgia Palmer, Sangjo Han, Linda Jeffery, Seung Tae Baek, Hyemi Lee, Young Sam Shim, Minho Lee, Lila Kim, Kyung Sun Heo, Eun Joo Noh, Ah Reum LeeYoung Joo Jang, Kyung Sook Chung, Shin Jung Choi, Jo Young Park, Youngwoo Park, Hwan Mook Kim, Song Kyu Park, Hae Joon Park, Eun Jung Kang, Hyong Bai Kim, Hyun Sam Kang, Hee Moon Park, Kyunghoon Kim, Kiwon Song, Kyung Bin Song, Paul Nurse, Kwang Lae Hoe

Research output: Contribution to journalArticlepeer-review

445 Citations (Scopus)

Abstract

We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeastthe only other eukaryote for which a comprehensive knockout library existsrevealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.

Original languageEnglish
Pages (from-to)617-623
Number of pages7
JournalNature Biotechnology
Volume28
Issue number6
DOIs
Publication statusPublished - 2010 Jun

Bibliographical note

Funding Information:
Y.-D. Kim, S. Park, H.-J. Lee, J.-H. Ahn, Y.-S. Kil, S.-Y. Park, J.-H. Lim, J.-H. Song, Y.-K. Ryoo, J.-Y. Kim, M.-J. Oh, S. Kong, J. Ahn, N. Sun, N. Peat, R. Mandeville and J.-J. Li. We also thank J.-H. Roe and W.-K. Huh for reading this manuscript and for their insightful comments and O. Nielsen for his patience with the many requests for pON177. This work was supported by the intramural research program of KRIBB (Mission 2007), the Chemical Genomics Research Program and the 21st Century Frontier Research Program from the Ministry of Education, Science and Technology (MOEST) of Korea. This work was also supported by Bioneer Corp., The Wellcome Trust, Cancer Research UK, The Breast Cancer Research Foundation (BCRF) and The Rockefeller University.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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