Analysis of age-related changes in the functional morphologies of salivary glands in mice

Jeong Seok Choi, In Suh Park, Seok Ki Kim, Jae Yol Lim, Young Mo Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background and Objectives Salivary glands in the elderly commonly exhibit salivary dysfunction resulting dry mouth, poor oral hygiene, and dental caries. However, in vivo changes of salivary glands during aging have not been well documented in the literature. This study was undertaken to determine age-related morphometric and functional changes of salivary glands using an aging mouse model. Methods Male C57BL/6 mice were divided into three groups, group A (10 weeks old; n = 10), group B (30 weeks old; n = 10), and group C (90 weeks old; n = 10). Body weights, salivary gland weights, salivary flow rates, and salivary lag times were measured and compared. Histomorphometric examinations and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed. In addition, changes in salivary uptake and excretion were observed by single-photon emission computed tomography (SPECT). Results Body and gland weights increased with age. Gland weight was significantly higher in group B than in groups A and C. Salivary lag time was significantly greater in group C than in groups A and B, and salivary flow rate was significantly greater in group B than in groups A and C. Histologic evaluations exhibited acinar cell atrophy, cytoplasmic vacuolization, lymphocyte infiltration, small mucin component and more periductal fibrosis in salivary glands of group C. TUNEL assays revealed that apoptotic salivary epithelial cells were significantly more numerous in group C than in groups A and B. 99mTc-pertechnetate excretion rate was significantly lower in group C than in groups A and B in SPECT. Conclusion Various morphometric and histopathological changes were observed in the salivary glands of aging mouse as well as relevant functional alterations, such as, decreased saliva production and excretion. Increased number of apoptotic salivary epithelial cells may contribute to the observed functional deterioration.

Original languageEnglish
Pages (from-to)1635-1642
Number of pages8
JournalArchives of Oral Biology
Volume58
Issue number11
DOIs
Publication statusPublished - 2013 Sep 18

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Salivary Glands
Single-Photon Emission-Computed Tomography
Epithelial Cells
Body Weight
Sodium Pertechnetate Tc 99m
Weights and Measures
DNA Nucleotidylexotransferase
Acinar Cells
Oral Hygiene
Dental Caries
Mucins
Transferases
Inbred C57BL Mouse
Saliva
Atrophy
Mouth
Fibrosis
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Choi, Jeong Seok ; Park, In Suh ; Kim, Seok Ki ; Lim, Jae Yol ; Kim, Young Mo. / Analysis of age-related changes in the functional morphologies of salivary glands in mice. In: Archives of Oral Biology. 2013 ; Vol. 58, No. 11. pp. 1635-1642.
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Analysis of age-related changes in the functional morphologies of salivary glands in mice. / Choi, Jeong Seok; Park, In Suh; Kim, Seok Ki; Lim, Jae Yol; Kim, Young Mo.

In: Archives of Oral Biology, Vol. 58, No. 11, 18.09.2013, p. 1635-1642.

Research output: Contribution to journalArticle

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T1 - Analysis of age-related changes in the functional morphologies of salivary glands in mice

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AU - Park, In Suh

AU - Kim, Seok Ki

AU - Lim, Jae Yol

AU - Kim, Young Mo

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N2 - Background and Objectives Salivary glands in the elderly commonly exhibit salivary dysfunction resulting dry mouth, poor oral hygiene, and dental caries. However, in vivo changes of salivary glands during aging have not been well documented in the literature. This study was undertaken to determine age-related morphometric and functional changes of salivary glands using an aging mouse model. Methods Male C57BL/6 mice were divided into three groups, group A (10 weeks old; n = 10), group B (30 weeks old; n = 10), and group C (90 weeks old; n = 10). Body weights, salivary gland weights, salivary flow rates, and salivary lag times were measured and compared. Histomorphometric examinations and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed. In addition, changes in salivary uptake and excretion were observed by single-photon emission computed tomography (SPECT). Results Body and gland weights increased with age. Gland weight was significantly higher in group B than in groups A and C. Salivary lag time was significantly greater in group C than in groups A and B, and salivary flow rate was significantly greater in group B than in groups A and C. Histologic evaluations exhibited acinar cell atrophy, cytoplasmic vacuolization, lymphocyte infiltration, small mucin component and more periductal fibrosis in salivary glands of group C. TUNEL assays revealed that apoptotic salivary epithelial cells were significantly more numerous in group C than in groups A and B. 99mTc-pertechnetate excretion rate was significantly lower in group C than in groups A and B in SPECT. Conclusion Various morphometric and histopathological changes were observed in the salivary glands of aging mouse as well as relevant functional alterations, such as, decreased saliva production and excretion. Increased number of apoptotic salivary epithelial cells may contribute to the observed functional deterioration.

AB - Background and Objectives Salivary glands in the elderly commonly exhibit salivary dysfunction resulting dry mouth, poor oral hygiene, and dental caries. However, in vivo changes of salivary glands during aging have not been well documented in the literature. This study was undertaken to determine age-related morphometric and functional changes of salivary glands using an aging mouse model. Methods Male C57BL/6 mice were divided into three groups, group A (10 weeks old; n = 10), group B (30 weeks old; n = 10), and group C (90 weeks old; n = 10). Body weights, salivary gland weights, salivary flow rates, and salivary lag times were measured and compared. Histomorphometric examinations and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed. In addition, changes in salivary uptake and excretion were observed by single-photon emission computed tomography (SPECT). Results Body and gland weights increased with age. Gland weight was significantly higher in group B than in groups A and C. Salivary lag time was significantly greater in group C than in groups A and B, and salivary flow rate was significantly greater in group B than in groups A and C. Histologic evaluations exhibited acinar cell atrophy, cytoplasmic vacuolization, lymphocyte infiltration, small mucin component and more periductal fibrosis in salivary glands of group C. TUNEL assays revealed that apoptotic salivary epithelial cells were significantly more numerous in group C than in groups A and B. 99mTc-pertechnetate excretion rate was significantly lower in group C than in groups A and B in SPECT. Conclusion Various morphometric and histopathological changes were observed in the salivary glands of aging mouse as well as relevant functional alterations, such as, decreased saliva production and excretion. Increased number of apoptotic salivary epithelial cells may contribute to the observed functional deterioration.

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