Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins

Heon Yung Gee, Joo Young Kim, Min Goo Lee

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is a polytopic transmembrane protein that functions as a cAMP-activated anion channel at the apical membrane of epithelial cells. Mutations in CFTR cause cystic fibrosis and are also associated with monosymptomatic diseases in the lung, pancreas, intestines, and vas deferens. Many disease-causing CFTR mutations, including the deletion of a single phenylalanine residue at position 508 (?F508-CFTR), result in protein misfolding and trafficking defects. Therefore, intracellular trafficking of wild-type and mutant CFTR has been studied extensively, and results from these studies significantly contribute to our general understanding of mechanisms involved in the cell-surface trafficking of membrane proteins. CFTR is a glycoprotein that undergoes complex N-glycosylation as it passes through Golgi-mediated conventional exocytosis. Interestingly, results from recent studies revealed that CFTR and other membrane proteins can reach the plasma membrane via an unconventional alternative route that bypasses Golgi in specific cellular conditions. Here, we describe methods that have been used to investigate the conventional and unconventional surface trafficking of CFTR. With appropriate modifications, the protocols described in this chapter can also be applied to studies investigating the intracellular trafficking of other plasma membrane proteins.

Original languageEnglish
Title of host publicationMembrane Trafficking
Subtitle of host publicationSecond Edition
PublisherSpringer New York
Pages137-154
Number of pages18
ISBN (Electronic)9781493923090
ISBN (Print)9781493923083
DOIs
Publication statusPublished - 2015 Feb 21

Fingerprint

Cystic Fibrosis Transmembrane Conductance Regulator
Membrane Proteins
Cell membranes
Cell Membrane
Glycosylation
Vas Deferens
Sequence Deletion
Exocytosis
Protein Transport
Phenylalanine
Cystic Fibrosis
Lung Diseases
Intestines
Anions
Blood Proteins
Pancreas
Glycoproteins
Proteins
Epithelial Cells
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gee, H. Y., Kim, J. Y., & Lee, M. G. (2015). Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins. In Membrane Trafficking: Second Edition (pp. 137-154). Springer New York. https://doi.org/10.1007/978-1-4939-2187-4_11
Gee, Heon Yung ; Kim, Joo Young ; Lee, Min Goo. / Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins. Membrane Trafficking: Second Edition. Springer New York, 2015. pp. 137-154
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Gee, HY, Kim, JY & Lee, MG 2015, Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins. in Membrane Trafficking: Second Edition. Springer New York, pp. 137-154. https://doi.org/10.1007/978-1-4939-2187-4_11

Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins. / Gee, Heon Yung; Kim, Joo Young; Lee, Min Goo.

Membrane Trafficking: Second Edition. Springer New York, 2015. p. 137-154.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Gee HY, Kim JY, Lee MG. Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins. In Membrane Trafficking: Second Edition. Springer New York. 2015. p. 137-154 https://doi.org/10.1007/978-1-4939-2187-4_11