Analysis of density-dependent binding of glycans by lectins using carbohydrate microarrays

Xizhe Tian, Jaeyoung Pai, Injae Shin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To investigate the density-dependent binding of glycans by lectins using carbohydrate microarrays, a number of C-terminal hydrazide-conjugated neoglycopeptides with various valences and different spatial arrangements of the sugar ligands were prepared on a solid support. The synthetic strategy includes (1) assembly of alkyne-linked peptides possessing C-terminal hydrazide on a solid support, (2) coupling of azide-linked, unprotected sugars to the alkyne-linked peptides on the solid support utilizing click chemistry, and (3) release of the neoglycopeptides from the solid support. By using this synthetic methodology, sixty five neoglycopeptides with a valency ranging from 1 to 4 and different spatial arrangements of the carbohydrate ligands were generated. Carbohydrate microarrays were constructed by immobilizing the prepared neoglycopeptides on epoxide-derivatized glass slides and were used to analyze the density-dependent binding of glycans by lectins. The results of binding property determinations show that lectin binding is highly dependent on the surface glycan density.

Original languageEnglish
Pages (from-to)2052-2060
Number of pages9
JournalChemistry - An Asian Journal
Volume7
Issue number9
DOIs
Publication statusPublished - 2012 Sep

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Analysis of density-dependent binding of glycans by lectins using carbohydrate microarrays'. Together they form a unique fingerprint.

  • Cite this