Analysis of hedgehog signaling in mouse intraflagellar transport mutants.

Hyuk Wan Ko, Aimin Liu, Jonathan T. Eggenschwiler

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intraflagellar transport (IFT) has been studied for decades in model systems such as Chlamydomonas and Caenorhabditis elegans. More recently, IFT has been investigated using genetic approaches in mammals using the mouse as a model system. Through such studies, a new appreciation of the importance of IFT and cilia in mammalian signal transduction has emerged. Specifically, IFT has been shown to play a key role in controlling signaling by Sonic and Indian Hedgehog (Hh) ligands. The effects of mutations in IFT components on Sonic Hh signaling in the embryo are complex and differ depending on the nature of the components, alleles, and tissues examined. For this reason, we provide a basis for analyzing the phenotype as a guide for those investigators who study IFT in cell culture or use invertebrate systems and wish to extend their studies to include development of the mouse embryo. We provide an overview of Sonic Hh-dependent tissue patterning in the developing neural tube and limb buds, the two systems in which it has been studied most extensively, and we show examples of how this patterning is disrupted by mutations in mouse IFT components. 2009 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)347-369
Number of pages23
JournalMethods in cell biology
Volume93
DOIs
Publication statusPublished - 2009 Jan 1

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Hedgehogs
Chlamydomonas
Limb Buds
Mutation
Neural Tube
Cilia
Caenorhabditis elegans
Invertebrates
Embryonic Development
Mammals
Signal Transduction
Embryonic Structures
Cell Culture Techniques
Alleles
Research Personnel
Ligands
Phenotype

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Ko, Hyuk Wan ; Liu, Aimin ; Eggenschwiler, Jonathan T. / Analysis of hedgehog signaling in mouse intraflagellar transport mutants. In: Methods in cell biology. 2009 ; Vol. 93. pp. 347-369.
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Analysis of hedgehog signaling in mouse intraflagellar transport mutants. / Ko, Hyuk Wan; Liu, Aimin; Eggenschwiler, Jonathan T.

In: Methods in cell biology, Vol. 93, 01.01.2009, p. 347-369.

Research output: Contribution to journalArticle

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