Analysis of matrix metalloproteinase expression in the recurrence of superficial low grade urothelial bladder cancer

Young Deuk Choi, Hyun Soo Ahn, Nam Hoon Cho, Mee Kyung Lee, Sook Hee Kang, Won Jae Yang

Research output: Contribution to journalArticle


Purpose: Superficial bladder transitional cell carcinomas (TCCa) have high recurrence rates following endoscopic resection. Most of the parameters used for the prediction of a recurrence are associated with high grade TCCa. However, there are few valid parameters for prediction of the recurrence of low grade TCCa. We aimed to elucidate the prime factor of a recurrence of superficial low grade bladder TCCa. Materials and methods: Thirty cases of superficial low grade bladder TCCa were analyzed, 15 of which were recurred cases. Fresh frozen tumor and normal tissues were used for the analysis. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots analyses were subsequently performed to evaluate the validity of the molecules identified by a 0.12K cDNA array, including matrix metalloproteinase (MMP), oncogenes and cell-cycle-related genes. Results: On cDNA microarray analysis, MMP-1, 2, 9, 12 and 15, and transforming growth factor β1 (TGF β1), vascular endothelial growth factor (VEGF) and fos were found to be significantly upregulated in the recurred cases. From the real-time RT-PCR for the validation of those molecules, MMP-1 and 12, and TGF β1, VEGF and fos were significantly overexpressed in the recurred cases. Western blot showed significant overexpression of MMP-1 and 12, and TGF β1, VEGF and fos in the recurred cases. Conclusions: The overexpressions of MMP-1 and 12, and TGF β1, VEGF and fos may be associated with the recurrence of superficial low grade bladder TCCa.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalKorean Journal of Urology
Issue number4
Publication statusPublished - 2005 Apr 1


All Science Journal Classification (ASJC) codes

  • Urology

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