Analysis of matrix metalloproteinase mRNAs expressed in hepatocellular carcinoma cell lines

Joo Hyon Kim, Tae Han Kim, Jin Wook Jang, Yoon Jung Jang, Kyung Hwa Lee, Seung Taek Lee

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To analyze matrix metalloproteinase (MMP) mRNAs that are expressed in hepatocellular carcinoma cell lines, the kinds of MMP mRNAs were surveyed in HepG2 and Hep3B cells and normal liver by a reverse transcription-polymerase chain reaction using two degenerate primer pairs, derived from conserved domains of known MMPs. The level for each MMP mRNA was examined by Northern blot analysis in HepG2 and Hep3B cells, as well as in normal tissues. It was also examined by a reverse transcription-polymerase chain reaction analysis in 8 different hepatocellular carcinoma cell lines. MMP-2, MMP-14, and MMP-15 mRNAs were expressed at elevated levels in most of the cell lines studied, reflecting that these MMPs would play an important role in the invasion and metastasis of hepatocellular carcinoma. MMP-1, MMP-3, MMP-8, MMP-10, MMP-11, and MMP-13 mRNAs were also expressed in some or most of the cell lines. Interestingly, MMP-9 mRNA, as well as its polypeptide, was undetected in all of the cell lines studied. This implies that MMP-9, which was suggested as a tumor marker for hepatocellular carcinoma, would be expressed in stromal cells, rather than tumor cells. These results provide information for the basal levels of MMP mRNAs in various hepatocellular carcinoma cell lines. It will also facilitate study on the transcriptional regulation of each MMP mRNA by oncogenes.

Original languageEnglish
Pages (from-to)32-40
Number of pages9
JournalMolecules and cells
Volume12
Issue number1
Publication statusPublished - 2001 Aug 31

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Matrix Metalloproteinases
Hepatocellular Carcinoma
Cell Line
Messenger RNA
Matrix Metalloproteinase 9
Hep G2 Cells
Reverse Transcription
Matrix Metalloproteinase 15
Matrix Metalloproteinase 10
Matrix Metalloproteinase 11
Matrix Metalloproteinase 8
Matrix Metalloproteinase 13
Matrix Metalloproteinase 14
Matrix Metalloproteinase 3
Polymerase Chain Reaction
Matrix Metalloproteinase 1
Matrix Metalloproteinase 2
Tumor Biomarkers
Stromal Cells
Oncogenes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Kim, J. H., Kim, T. H., Jang, J. W., Jang, Y. J., Lee, K. H., & Lee, S. T. (2001). Analysis of matrix metalloproteinase mRNAs expressed in hepatocellular carcinoma cell lines. Molecules and cells, 12(1), 32-40.
Kim, Joo Hyon ; Kim, Tae Han ; Jang, Jin Wook ; Jang, Yoon Jung ; Lee, Kyung Hwa ; Lee, Seung Taek. / Analysis of matrix metalloproteinase mRNAs expressed in hepatocellular carcinoma cell lines. In: Molecules and cells. 2001 ; Vol. 12, No. 1. pp. 32-40.
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abstract = "To analyze matrix metalloproteinase (MMP) mRNAs that are expressed in hepatocellular carcinoma cell lines, the kinds of MMP mRNAs were surveyed in HepG2 and Hep3B cells and normal liver by a reverse transcription-polymerase chain reaction using two degenerate primer pairs, derived from conserved domains of known MMPs. The level for each MMP mRNA was examined by Northern blot analysis in HepG2 and Hep3B cells, as well as in normal tissues. It was also examined by a reverse transcription-polymerase chain reaction analysis in 8 different hepatocellular carcinoma cell lines. MMP-2, MMP-14, and MMP-15 mRNAs were expressed at elevated levels in most of the cell lines studied, reflecting that these MMPs would play an important role in the invasion and metastasis of hepatocellular carcinoma. MMP-1, MMP-3, MMP-8, MMP-10, MMP-11, and MMP-13 mRNAs were also expressed in some or most of the cell lines. Interestingly, MMP-9 mRNA, as well as its polypeptide, was undetected in all of the cell lines studied. This implies that MMP-9, which was suggested as a tumor marker for hepatocellular carcinoma, would be expressed in stromal cells, rather than tumor cells. These results provide information for the basal levels of MMP mRNAs in various hepatocellular carcinoma cell lines. It will also facilitate study on the transcriptional regulation of each MMP mRNA by oncogenes.",
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Kim, JH, Kim, TH, Jang, JW, Jang, YJ, Lee, KH & Lee, ST 2001, 'Analysis of matrix metalloproteinase mRNAs expressed in hepatocellular carcinoma cell lines', Molecules and cells, vol. 12, no. 1, pp. 32-40.

Analysis of matrix metalloproteinase mRNAs expressed in hepatocellular carcinoma cell lines. / Kim, Joo Hyon; Kim, Tae Han; Jang, Jin Wook; Jang, Yoon Jung; Lee, Kyung Hwa; Lee, Seung Taek.

In: Molecules and cells, Vol. 12, No. 1, 31.08.2001, p. 32-40.

Research output: Contribution to journalArticle

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