Analysis of nuclear high mobility group box 1 (HMGB1)-binding proteins in colon cancer cells

Clustering with proteins involved in secretion and extranuclear function

Hanna Lee, Nara Shin, Meiying Song, Un Beom Kang, Jeonghun Yeom, Cheolju Lee, Yeong Hee Ahn, Jong Shin Yoo, Young-Ki Paik, Hoguen Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

HMGB1 is a nuclear protein that is overexpressed and secreted in cancer cells. However, little is known about the roles of HMGB1 in the cytoplasm and secretory pathway in cancer cells. To clarify this aspect of HMGB1 function, we fractionated the cytoplasm of HCT116 colon cancer cells and used a proteomic approach to analyze cytoplasmic HMGB1-binding proteins. Pull-down experiments using recombinant HMGB1 protein as bait, followed by mass spectrometry analysis identified 162 interacting proteins. Among them were 74 proteins known to be localized exclusively to the extra-nuclear region, and 60 proteins known to be localized to both nuclear and extranuclear regions. The functions of these binding proteins include involvement in cell-cycle progression, cell proliferation, anti-apoptosis, and angiogenesis. In addition, nine of the identified proteins are related to protein translocation and secretion. These include annexin A2, myosin IC isoform a, myosin-9, and Ras-related protein Rab10, which are involved in unconventional protein secretion. Cytoplasmic HMGB1 was primarily associated with the lysosomal cytosol fraction and was colocalized with the lysosomal marker LAMP1. Our findings suggest that cytoplasmic HMGB1 binds to a number of molecules related to cancer progression and the unconventional secretory pathway.

Original languageEnglish
Pages (from-to)4661-4670
Number of pages10
JournalJournal of Proteome Research
Volume9
Issue number9
DOIs
Publication statusPublished - 2010 Sep 3

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Colonic Neoplasms
Cluster Analysis
Carrier Proteins
Cells
Secretory Pathway
Myosins
Proteins
Cytoplasm
Annexin A2
HMGB1 Protein
ras Proteins
Neoplasms
Protein Transport
Nuclear Proteins
Cytosol
Proteomics
Mass Spectrometry
Cell Cycle
Protein Isoforms
Cell proliferation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

Lee, Hanna ; Shin, Nara ; Song, Meiying ; Kang, Un Beom ; Yeom, Jeonghun ; Lee, Cheolju ; Ahn, Yeong Hee ; Yoo, Jong Shin ; Paik, Young-Ki ; Kim, Hoguen. / Analysis of nuclear high mobility group box 1 (HMGB1)-binding proteins in colon cancer cells : Clustering with proteins involved in secretion and extranuclear function. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 9. pp. 4661-4670.
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Analysis of nuclear high mobility group box 1 (HMGB1)-binding proteins in colon cancer cells : Clustering with proteins involved in secretion and extranuclear function. / Lee, Hanna; Shin, Nara; Song, Meiying; Kang, Un Beom; Yeom, Jeonghun; Lee, Cheolju; Ahn, Yeong Hee; Yoo, Jong Shin; Paik, Young-Ki; Kim, Hoguen.

In: Journal of Proteome Research, Vol. 9, No. 9, 03.09.2010, p. 4661-4670.

Research output: Contribution to journalArticle

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