Analysis of outer membrane proteins of Yersinia enterocolitica isolated from mountainspring water and pig

SungJae Shin, Joo Youn Park, In Soo Choi, Na Ri Shin, Deog Yong Lee, Young Wook Cho, Yong Ha Park, Han Sang Yoo

Research output: Contribution to journalArticle

Abstract

Yersinia enterocolitica causes various diseases in humans, including enteritis. The onset of such diseases is closely related with the expression of important virulence factors, particularly outer membrane proteins (OMPs). The expression of OMPs depends on several factors, including temperature, and origin, biotype and serotype of the bacteria. Recently, concerns over food safety have increased along with the demand for the development of sensitive, rapid, and pathogen-specific detection methods. To develop a suitable detection method for Y. enterocolitica isolated from Korean moutainspring water and pig feces, the OMP expression patterns were analyzed phenotypically and immunologically using 12 representative strains from 51 Y. enterocolitica Korean isolates. A 38-kDa OMP was commonly observed in all strains. However, additional OMPs were also observed in different biotypes and serotypes as well as bacterial origins, by incubating Y. Enterocolitica at a low temperature. The specificity of the 38-kDa OMP was confirmed by a Western blot analysis with antisera against Y. enterocolitica and Brucella abortus. The results, therefore, indicate that the 38-kDa OMP could be used as a marker for detecting Y. enterocolitica in the environment or for seromonitoring.

Original languageEnglish
Pages (from-to)674-678
Number of pages5
JournalJournal of microbiology and biotechnology
Volume12
Issue number4
Publication statusPublished - 2002 Aug 1

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Yersinia enterocolitica
Membrane Proteins
Swine
Water
Brucella abortus
Temperature
Enteritis
Food Safety
Virulence Factors
Feces
Immune Sera
Western Blotting
Bacteria

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Shin, S., Park, J. Y., Choi, I. S., Shin, N. R., Lee, D. Y., Cho, Y. W., ... Yoo, H. S. (2002). Analysis of outer membrane proteins of Yersinia enterocolitica isolated from mountainspring water and pig. Journal of microbiology and biotechnology, 12(4), 674-678.
Shin, SungJae ; Park, Joo Youn ; Choi, In Soo ; Shin, Na Ri ; Lee, Deog Yong ; Cho, Young Wook ; Park, Yong Ha ; Yoo, Han Sang. / Analysis of outer membrane proteins of Yersinia enterocolitica isolated from mountainspring water and pig. In: Journal of microbiology and biotechnology. 2002 ; Vol. 12, No. 4. pp. 674-678.
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abstract = "Yersinia enterocolitica causes various diseases in humans, including enteritis. The onset of such diseases is closely related with the expression of important virulence factors, particularly outer membrane proteins (OMPs). The expression of OMPs depends on several factors, including temperature, and origin, biotype and serotype of the bacteria. Recently, concerns over food safety have increased along with the demand for the development of sensitive, rapid, and pathogen-specific detection methods. To develop a suitable detection method for Y. enterocolitica isolated from Korean moutainspring water and pig feces, the OMP expression patterns were analyzed phenotypically and immunologically using 12 representative strains from 51 Y. enterocolitica Korean isolates. A 38-kDa OMP was commonly observed in all strains. However, additional OMPs were also observed in different biotypes and serotypes as well as bacterial origins, by incubating Y. Enterocolitica at a low temperature. The specificity of the 38-kDa OMP was confirmed by a Western blot analysis with antisera against Y. enterocolitica and Brucella abortus. The results, therefore, indicate that the 38-kDa OMP could be used as a marker for detecting Y. enterocolitica in the environment or for seromonitoring.",
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Shin, S, Park, JY, Choi, IS, Shin, NR, Lee, DY, Cho, YW, Park, YH & Yoo, HS 2002, 'Analysis of outer membrane proteins of Yersinia enterocolitica isolated from mountainspring water and pig', Journal of microbiology and biotechnology, vol. 12, no. 4, pp. 674-678.

Analysis of outer membrane proteins of Yersinia enterocolitica isolated from mountainspring water and pig. / Shin, SungJae; Park, Joo Youn; Choi, In Soo; Shin, Na Ri; Lee, Deog Yong; Cho, Young Wook; Park, Yong Ha; Yoo, Han Sang.

In: Journal of microbiology and biotechnology, Vol. 12, No. 4, 01.08.2002, p. 674-678.

Research output: Contribution to journalArticle

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