Analysis of phospholipids using an open-tubular capillary column with a monolithic layer of molecularly imprinted polymer in capillary electrochromatography-electrospray ionization-tandem mass spectrometry

Rin Jang, Ki Hun Kim, Shabi Abbas Zaidi, Won Jo Cheong, Myeong Hee Moon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this study, an open-tubular capillary electrochromatography (OT-CEC) column with a monolithic layer of molecularly imprinted polymer (MIP) based on methacrylic acid, ethylene glycol dimethacrylate, and 4-styrenesulfonic acid was utilized for the simultaneous separation and characterization of phospholipid (PL) molecular structures by interfacing with electrospray ionization-tandem mass spectrometry (ESI-MS-MS). Introducing an MIP-based monolith along with charged species at the OT column made it possible to separate PL molecules based on differences in head groups and acyl chain lengths in CEC. For the interface of OT-CEC with ESI-MS-MS, a simple nanospray interface utilizing a sheath flow was developed and the resulting OT-CEC-ESI-MS-MS was able to separate PL standards (phosphatidylserines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidic acid, and lysophosphatidylglycerols). The developed method was applied to human urinary lipid extracts, and resulted in the separation and structural identification of 18 molecules by data-dependent collision-induced dissociation.

Original languageEnglish
Pages (from-to)2167-2173
Number of pages7
JournalElectrophoresis
Volume32
Issue number16
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Capillary Electrochromatography
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Tandem Mass Spectrometry
Mass spectrometry
Phospholipids
Polymers
Phosphatidylethanolamines
Phosphatidylglycerols
Phosphatidic Acids
Molecules
Phosphatidylserines
Molecular Structure
Chain length
Molecular structure
Head
Lipids
Acids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

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Analysis of phospholipids using an open-tubular capillary column with a monolithic layer of molecularly imprinted polymer in capillary electrochromatography-electrospray ionization-tandem mass spectrometry. / Jang, Rin; Kim, Ki Hun; Zaidi, Shabi Abbas; Cheong, Won Jo; Moon, Myeong Hee.

In: Electrophoresis, Vol. 32, No. 16, 01.08.2011, p. 2167-2173.

Research output: Contribution to journalArticle

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