Analysis of polyamines as carbamoyl derivatives in urine and serum by liquid chromatography-tandem mass spectrometry

Jeong Ah Byun, Sang Hee Lee, Byung Hwa Jung, Man Ho Choi, Myeong Hee Moon, Bong Chul Chung

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A quantitative analysis of polyamines in urine and serum by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is described. The polyamines were carbamylated with isobutyl chloroformate, extracted with diethyl ether under pH 9.0, and analyzed by LC-MS/MS with single reaction monitoring mode. The limit of quantification was 1 ng/mL based on a signal-to-noise ratio >3, and the correlation coefficient (r2) for the calibration curves was >0.99 for both urine and serum samples. The present method was applied to urine and serum samples from 30 breast cancer patients and 30 normal female controls. There was no significant difference in the urinary polyamine levels between breast cancer patients and controls. However, 1,3-diaminopropane, putrescine, spermine and N-acetylspermidine levels in serum increased in breast cancer patients. These four serum polyamines may be a good index to study both production and metabolism of polyamines, and a useful tool in assessment of the polyamine status of breast cancer patients.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalBiomedical Chromatography
Volume22
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

Liquid chromatography
Polyamines
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Urine
Derivatives
Serum
Breast Neoplasms
Putrescine
Spermine
Signal-To-Noise Ratio
Metabolism
Ether
Calibration
Signal to noise ratio
Monitoring
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Byun, Jeong Ah ; Lee, Sang Hee ; Jung, Byung Hwa ; Choi, Man Ho ; Moon, Myeong Hee ; Chung, Bong Chul. / Analysis of polyamines as carbamoyl derivatives in urine and serum by liquid chromatography-tandem mass spectrometry. In: Biomedical Chromatography. 2008 ; Vol. 22, No. 1. pp. 73-80.
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Analysis of polyamines as carbamoyl derivatives in urine and serum by liquid chromatography-tandem mass spectrometry. / Byun, Jeong Ah; Lee, Sang Hee; Jung, Byung Hwa; Choi, Man Ho; Moon, Myeong Hee; Chung, Bong Chul.

In: Biomedical Chromatography, Vol. 22, No. 1, 01.01.2008, p. 73-80.

Research output: Contribution to journalArticle

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