TY - JOUR
T1 - Analysis of the envelope (E) protein gene of tick-borne encephalitis viruses isolated in South Korea
AU - Yun, Seok Min
AU - Kim, Su Yeon
AU - Han, Myung Guk
AU - Jeong, Young Eui
AU - Yong, Tai Soon
AU - Lee, Chan Hee
AU - Ju, Young Ran
PY - 2009/6/1
Y1 - 2009/6/1
N2 - We determined the nucleotide and deduced amino acid sequences of the complete envelope (E) protein gene of the five tick-borne encephalitis virus (TBEV) strains KrM 93, KrM 213, KrM 215, KrM 216, and KrM 219, isolated from wild rodents in South Korea. We analyzed genetic variability within the isolates and compared them with 13 other TBEV strains. The complete E protein genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), cloned into pGEM-T vectors, and sequenced. The five isolates were similar to the Western subtype in nucleotide and deduced amino acid sequences (97%-99% identity) and by phylogenetic analysis. The deduced amino acid alignments had 13 unique amino acids, as in the Western subtypes. Among the signature amino acids, those at positions 206 and 317 were unique to each subtype. We were also able to identify amino acid substitutions in each of the three domains when comparing the 5 Korean isolates with the 13 other TBEV strains. Thus, we confirmed that the 5 Korean isolates belong to the Western subtype. These data will provide useful information for the development of an effective recombinant vaccine.
AB - We determined the nucleotide and deduced amino acid sequences of the complete envelope (E) protein gene of the five tick-borne encephalitis virus (TBEV) strains KrM 93, KrM 213, KrM 215, KrM 216, and KrM 219, isolated from wild rodents in South Korea. We analyzed genetic variability within the isolates and compared them with 13 other TBEV strains. The complete E protein genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), cloned into pGEM-T vectors, and sequenced. The five isolates were similar to the Western subtype in nucleotide and deduced amino acid sequences (97%-99% identity) and by phylogenetic analysis. The deduced amino acid alignments had 13 unique amino acids, as in the Western subtypes. Among the signature amino acids, those at positions 206 and 317 were unique to each subtype. We were also able to identify amino acid substitutions in each of the three domains when comparing the 5 Korean isolates with the 13 other TBEV strains. Thus, we confirmed that the 5 Korean isolates belong to the Western subtype. These data will provide useful information for the development of an effective recombinant vaccine.
UR - http://www.scopus.com/inward/record.url?scp=68049143205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68049143205&partnerID=8YFLogxK
U2 - 10.1089/vbz.2008.0085
DO - 10.1089/vbz.2008.0085
M3 - Article
C2 - 19480604
AN - SCOPUS:68049143205
SN - 1530-3667
VL - 9
SP - 287
EP - 293
JO - Vector-Borne and Zoonotic Diseases
JF - Vector-Borne and Zoonotic Diseases
IS - 3
ER -