Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells

SunOch Yoon, Tae Joo Jeon, Joon Seong Park, Yong Hoon Ryu, Jae Hoon Lee, Jung Sik Yoo, Jae Keun Kim, Dong Sup Yoon, Eun Ji Oh

Research output: Contribution to journalArticle

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Abstract

PURPOSE OF THE REPORT: Extrahepatic bile duct (EHD) cancer varies in uptake of FDG. The aim of the present study was to determine the role of glucose transporter (GLUT) 1 and hexokinase (HK) 2 in the glucose metabolism of EHD cancer cells using immunohistochemistry and F-FDG PET/CT. METHODS: Twenty-six patients with EHD cancer who underwent baseline PET/CT and surgery were studied. Biopsies were immunohistochemically analyzed using antibodies against GLUT1 and HK2, and the expression was scored from 0 to 4 according to the percentage of stained cells. SUV and tumor-to-liver ratio (T/Lratio) were obtained from F-FDG PET/CT data. SUV and T/Lratio and GLUT1 and HK2 expression were compared with histological grades and tumor locations (proximal and distal EHD) to correlate glucose metabolism with the expression of GLUT1 and HK2. RESULTS: SUV, T/Lratio, and GLUT1 and HK2 expression did not differ as a function of histological grade and tumor location. GLUT1 and HK2 were expressed in 20 (76.9%) and 22 (84.6%) of 26 tumor biopsies, respectively. The GLUT1 score, SUV, and T/Lratio increased, and the GLUT1 score, but not the HK2 score, correlated significantly with SUV (ρ = 0.648) and T/Lratio (ρ = 0.703). There was no direct correlation between the expression of GLUT1 and that of HK2 (ρ = 0.2046, P = 0.3161). CONCLUSIONS: Although GLUT1 and HK2 regulate intracellular accumulation of FDG in many cancers, only GLUT1 expression was correlated with FDG uptake by EHD cancers.

Original languageEnglish
Pages (from-to)e178-e182
JournalClinical Nuclear Medicine
Volume40
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1

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Bile Duct Neoplasms
Extrahepatic Bile Ducts
Hexokinase
Facilitative Glucose Transport Proteins
Glucose
Liver
Neoplasms
Biopsy
Immunohistochemistry
Antibodies

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Yoon, SunOch ; Jeon, Tae Joo ; Park, Joon Seong ; Ryu, Yong Hoon ; Lee, Jae Hoon ; Yoo, Jung Sik ; Kim, Jae Keun ; Yoon, Dong Sup ; Oh, Eun Ji. / Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells. In: Clinical Nuclear Medicine. 2015 ; Vol. 40, No. 3. pp. e178-e182.
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title = "Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells",
abstract = "PURPOSE OF THE REPORT: Extrahepatic bile duct (EHD) cancer varies in uptake of FDG. The aim of the present study was to determine the role of glucose transporter (GLUT) 1 and hexokinase (HK) 2 in the glucose metabolism of EHD cancer cells using immunohistochemistry and F-FDG PET/CT. METHODS: Twenty-six patients with EHD cancer who underwent baseline PET/CT and surgery were studied. Biopsies were immunohistochemically analyzed using antibodies against GLUT1 and HK2, and the expression was scored from 0 to 4 according to the percentage of stained cells. SUV and tumor-to-liver ratio (T/Lratio) were obtained from F-FDG PET/CT data. SUV and T/Lratio and GLUT1 and HK2 expression were compared with histological grades and tumor locations (proximal and distal EHD) to correlate glucose metabolism with the expression of GLUT1 and HK2. RESULTS: SUV, T/Lratio, and GLUT1 and HK2 expression did not differ as a function of histological grade and tumor location. GLUT1 and HK2 were expressed in 20 (76.9{\%}) and 22 (84.6{\%}) of 26 tumor biopsies, respectively. The GLUT1 score, SUV, and T/Lratio increased, and the GLUT1 score, but not the HK2 score, correlated significantly with SUV (ρ = 0.648) and T/Lratio (ρ = 0.703). There was no direct correlation between the expression of GLUT1 and that of HK2 (ρ = 0.2046, P = 0.3161). CONCLUSIONS: Although GLUT1 and HK2 regulate intracellular accumulation of FDG in many cancers, only GLUT1 expression was correlated with FDG uptake by EHD cancers.",
author = "SunOch Yoon and Jeon, {Tae Joo} and Park, {Joon Seong} and Ryu, {Yong Hoon} and Lee, {Jae Hoon} and Yoo, {Jung Sik} and Kim, {Jae Keun} and Yoon, {Dong Sup} and Oh, {Eun Ji}",
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Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells. / Yoon, SunOch; Jeon, Tae Joo; Park, Joon Seong; Ryu, Yong Hoon; Lee, Jae Hoon; Yoo, Jung Sik; Kim, Jae Keun; Yoon, Dong Sup; Oh, Eun Ji.

In: Clinical Nuclear Medicine, Vol. 40, No. 3, 01.01.2015, p. e178-e182.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells

AU - Yoon, SunOch

AU - Jeon, Tae Joo

AU - Park, Joon Seong

AU - Ryu, Yong Hoon

AU - Lee, Jae Hoon

AU - Yoo, Jung Sik

AU - Kim, Jae Keun

AU - Yoon, Dong Sup

AU - Oh, Eun Ji

PY - 2015/1/1

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N2 - PURPOSE OF THE REPORT: Extrahepatic bile duct (EHD) cancer varies in uptake of FDG. The aim of the present study was to determine the role of glucose transporter (GLUT) 1 and hexokinase (HK) 2 in the glucose metabolism of EHD cancer cells using immunohistochemistry and F-FDG PET/CT. METHODS: Twenty-six patients with EHD cancer who underwent baseline PET/CT and surgery were studied. Biopsies were immunohistochemically analyzed using antibodies against GLUT1 and HK2, and the expression was scored from 0 to 4 according to the percentage of stained cells. SUV and tumor-to-liver ratio (T/Lratio) were obtained from F-FDG PET/CT data. SUV and T/Lratio and GLUT1 and HK2 expression were compared with histological grades and tumor locations (proximal and distal EHD) to correlate glucose metabolism with the expression of GLUT1 and HK2. RESULTS: SUV, T/Lratio, and GLUT1 and HK2 expression did not differ as a function of histological grade and tumor location. GLUT1 and HK2 were expressed in 20 (76.9%) and 22 (84.6%) of 26 tumor biopsies, respectively. The GLUT1 score, SUV, and T/Lratio increased, and the GLUT1 score, but not the HK2 score, correlated significantly with SUV (ρ = 0.648) and T/Lratio (ρ = 0.703). There was no direct correlation between the expression of GLUT1 and that of HK2 (ρ = 0.2046, P = 0.3161). CONCLUSIONS: Although GLUT1 and HK2 regulate intracellular accumulation of FDG in many cancers, only GLUT1 expression was correlated with FDG uptake by EHD cancers.

AB - PURPOSE OF THE REPORT: Extrahepatic bile duct (EHD) cancer varies in uptake of FDG. The aim of the present study was to determine the role of glucose transporter (GLUT) 1 and hexokinase (HK) 2 in the glucose metabolism of EHD cancer cells using immunohistochemistry and F-FDG PET/CT. METHODS: Twenty-six patients with EHD cancer who underwent baseline PET/CT and surgery were studied. Biopsies were immunohistochemically analyzed using antibodies against GLUT1 and HK2, and the expression was scored from 0 to 4 according to the percentage of stained cells. SUV and tumor-to-liver ratio (T/Lratio) were obtained from F-FDG PET/CT data. SUV and T/Lratio and GLUT1 and HK2 expression were compared with histological grades and tumor locations (proximal and distal EHD) to correlate glucose metabolism with the expression of GLUT1 and HK2. RESULTS: SUV, T/Lratio, and GLUT1 and HK2 expression did not differ as a function of histological grade and tumor location. GLUT1 and HK2 were expressed in 20 (76.9%) and 22 (84.6%) of 26 tumor biopsies, respectively. The GLUT1 score, SUV, and T/Lratio increased, and the GLUT1 score, but not the HK2 score, correlated significantly with SUV (ρ = 0.648) and T/Lratio (ρ = 0.703). There was no direct correlation between the expression of GLUT1 and that of HK2 (ρ = 0.2046, P = 0.3161). CONCLUSIONS: Although GLUT1 and HK2 regulate intracellular accumulation of FDG in many cancers, only GLUT1 expression was correlated with FDG uptake by EHD cancers.

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