Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three-dimensional structure

Jin Kyu Rhee, Do Yun Kim, Dae Gyun Ahn, Jung Hyuk Yun, Seung Hwan Jang, Hang Cheol Shin, Hyun Soo Cho, Jae Gu Pan, Jong-Won Oh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The three-dimensional (3D) structure of the hyperthermophilic esterase EstE1 was constructed by homology modeling using Archaeoglobus fulgidus esterase as a reference, and the thermostability-structure relationship was analyzed. Our results verified the predicted 3D structure of EstE1 and identified the ion pair networks and hydrophobic interactions that are critical determinants for the thermostability of EstE1.

Original languageEnglish
Pages (from-to)3021-3025
Number of pages5
JournalApplied and Environmental Microbiology
Volume72
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1

Fingerprint

Esterases
thermal stability
homology
esterases
Archaeoglobus
Archaeoglobus fulgidus
ion
hydrophobic bonding
Hydrophobic and Hydrophilic Interactions
modeling
Ions
ions
analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Rhee, Jin Kyu ; Kim, Do Yun ; Ahn, Dae Gyun ; Yun, Jung Hyuk ; Jang, Seung Hwan ; Shin, Hang Cheol ; Cho, Hyun Soo ; Pan, Jae Gu ; Oh, Jong-Won. / Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three-dimensional structure. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 4. pp. 3021-3025.
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Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three-dimensional structure. / Rhee, Jin Kyu; Kim, Do Yun; Ahn, Dae Gyun; Yun, Jung Hyuk; Jang, Seung Hwan; Shin, Hang Cheol; Cho, Hyun Soo; Pan, Jae Gu; Oh, Jong-Won.

In: Applied and Environmental Microbiology, Vol. 72, No. 4, 01.04.2006, p. 3021-3025.

Research output: Contribution to journalArticle

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