Analysis of three bacterial strains isolated from symptomatic plants in Australia

Jihyun F. Kim, E. R. Garr, D. W. Bauer, S. V. Beer, H. L. Gustafson, E. A. Momol, M. T. Momol, H. S. Aldwinckle, J. L. Vanneste

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In April, 1997, bacterial strains were isolated in New Zealand from tissues of cotoneaster plants with typical symptoms of fire blight, which had grown in the Royal Botanical Gardens in Melbourne, Australia. To determine the relationship among the isolated strains and known strains of E. amylovora, three Australian strains, two strains from Maloideae hosts in New Zealand and Ea321 (CNBP 1367; ATCC 49947), a weakly virulent strain from Belgium, were subjected to nutritional, molecular and pathogenicity analysis under blind coding. Analysis of their carbon utilization pattern by Biolog, suggested that the three Australian strains are E. amylovora. Polymerase chain reaction (PCR) of the Australian strains using primers (Bereswill et al., 1992) derived from pEA29 resulted in amplified bands, whose sizes correspond to those of the PCR products of most E. amylovora strains. Cleaved Amplified Polymorphic Sequences (CAPS) of 48 diverse E. amylovora strains were determined using two regions of the hrp gene cluster. The CAPS profiles of the Australian strains were identical to those of strains pathogenic to plants in the subfamily Maloideae, and present in North America, Europe, the Middle East and New Zealand, but different from strains pathogenic to Rubus species and those isolated in Hokkaido, Japan. PCR products of the intergenic region of the 1 6S-23S rDNA also indicated that the Australian isolates cluster with Maloideae-infecting strains of E. amylovora. Succulent shoot tips of apple and pear cultivars were inoculated with ca. lO cfu/ml of the six strains. Two Australian strains (now ICMP 13298 and 13299) infected all twelve apple and all three pear cultivars inoculated. The third (now ICMP 13293) infected three of twelve apple but all three pear cultivars inoculated. In general, the virulence of the latter Australian strain, as judged by percent lesion length, was less than that of the first two Australian strains and was similar to that of one New Zealand strain, and strain Ea321. Our results support reports that fire blight occurred in Australia, and indicate that the Australian strains are similar to Maloideae-infecting strains of E. amylovora analyzed previously from North America, Europe and the Middle East.

Original languageEnglish
Title of host publicationVIII International Workshop on Fire Blight
EditorsM.T. Momol, H. Saygili
Pages149-154
Number of pages6
Publication statusPublished - 1999 Dec 1

Publication series

NameActa Horticulturae
Volume489
ISSN (Print)0567-7572

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pears
apples
Erwinia amylovora
polymerase chain reaction
Middle East
cultivars
Cotoneaster
Japan
lesions (plant)
Rubus
botanical gardens
intergenic DNA
multigene family
Belgium
signs and symptoms (plants)
virulence
pathogenicity
carbon
virulent strains
shoot tips

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Kim, J. F., Garr, E. R., Bauer, D. W., Beer, S. V., Gustafson, H. L., Momol, E. A., ... Vanneste, J. L. (1999). Analysis of three bacterial strains isolated from symptomatic plants in Australia. In M. T. Momol, & H. Saygili (Eds.), VIII International Workshop on Fire Blight (pp. 149-154). (Acta Horticulturae; Vol. 489).
Kim, Jihyun F. ; Garr, E. R. ; Bauer, D. W. ; Beer, S. V. ; Gustafson, H. L. ; Momol, E. A. ; Momol, M. T. ; Aldwinckle, H. S. ; Vanneste, J. L. / Analysis of three bacterial strains isolated from symptomatic plants in Australia. VIII International Workshop on Fire Blight. editor / M.T. Momol ; H. Saygili. 1999. pp. 149-154 (Acta Horticulturae).
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abstract = "In April, 1997, bacterial strains were isolated in New Zealand from tissues of cotoneaster plants with typical symptoms of fire blight, which had grown in the Royal Botanical Gardens in Melbourne, Australia. To determine the relationship among the isolated strains and known strains of E. amylovora, three Australian strains, two strains from Maloideae hosts in New Zealand and Ea321 (CNBP 1367; ATCC 49947), a weakly virulent strain from Belgium, were subjected to nutritional, molecular and pathogenicity analysis under blind coding. Analysis of their carbon utilization pattern by Biolog, suggested that the three Australian strains are E. amylovora. Polymerase chain reaction (PCR) of the Australian strains using primers (Bereswill et al., 1992) derived from pEA29 resulted in amplified bands, whose sizes correspond to those of the PCR products of most E. amylovora strains. Cleaved Amplified Polymorphic Sequences (CAPS) of 48 diverse E. amylovora strains were determined using two regions of the hrp gene cluster. The CAPS profiles of the Australian strains were identical to those of strains pathogenic to plants in the subfamily Maloideae, and present in North America, Europe, the Middle East and New Zealand, but different from strains pathogenic to Rubus species and those isolated in Hokkaido, Japan. PCR products of the intergenic region of the 1 6S-23S rDNA also indicated that the Australian isolates cluster with Maloideae-infecting strains of E. amylovora. Succulent shoot tips of apple and pear cultivars were inoculated with ca. lO cfu/ml of the six strains. Two Australian strains (now ICMP 13298 and 13299) infected all twelve apple and all three pear cultivars inoculated. The third (now ICMP 13293) infected three of twelve apple but all three pear cultivars inoculated. In general, the virulence of the latter Australian strain, as judged by percent lesion length, was less than that of the first two Australian strains and was similar to that of one New Zealand strain, and strain Ea321. Our results support reports that fire blight occurred in Australia, and indicate that the Australian strains are similar to Maloideae-infecting strains of E. amylovora analyzed previously from North America, Europe and the Middle East.",
author = "Kim, {Jihyun F.} and Garr, {E. R.} and Bauer, {D. W.} and Beer, {S. V.} and Gustafson, {H. L.} and Momol, {E. A.} and Momol, {M. T.} and Aldwinckle, {H. S.} and Vanneste, {J. L.}",
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Kim, JF, Garr, ER, Bauer, DW, Beer, SV, Gustafson, HL, Momol, EA, Momol, MT, Aldwinckle, HS & Vanneste, JL 1999, Analysis of three bacterial strains isolated from symptomatic plants in Australia. in MT Momol & H Saygili (eds), VIII International Workshop on Fire Blight. Acta Horticulturae, vol. 489, pp. 149-154.

Analysis of three bacterial strains isolated from symptomatic plants in Australia. / Kim, Jihyun F.; Garr, E. R.; Bauer, D. W.; Beer, S. V.; Gustafson, H. L.; Momol, E. A.; Momol, M. T.; Aldwinckle, H. S.; Vanneste, J. L.

VIII International Workshop on Fire Blight. ed. / M.T. Momol; H. Saygili. 1999. p. 149-154 (Acta Horticulturae; Vol. 489).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - In April, 1997, bacterial strains were isolated in New Zealand from tissues of cotoneaster plants with typical symptoms of fire blight, which had grown in the Royal Botanical Gardens in Melbourne, Australia. To determine the relationship among the isolated strains and known strains of E. amylovora, three Australian strains, two strains from Maloideae hosts in New Zealand and Ea321 (CNBP 1367; ATCC 49947), a weakly virulent strain from Belgium, were subjected to nutritional, molecular and pathogenicity analysis under blind coding. Analysis of their carbon utilization pattern by Biolog, suggested that the three Australian strains are E. amylovora. Polymerase chain reaction (PCR) of the Australian strains using primers (Bereswill et al., 1992) derived from pEA29 resulted in amplified bands, whose sizes correspond to those of the PCR products of most E. amylovora strains. Cleaved Amplified Polymorphic Sequences (CAPS) of 48 diverse E. amylovora strains were determined using two regions of the hrp gene cluster. The CAPS profiles of the Australian strains were identical to those of strains pathogenic to plants in the subfamily Maloideae, and present in North America, Europe, the Middle East and New Zealand, but different from strains pathogenic to Rubus species and those isolated in Hokkaido, Japan. PCR products of the intergenic region of the 1 6S-23S rDNA also indicated that the Australian isolates cluster with Maloideae-infecting strains of E. amylovora. Succulent shoot tips of apple and pear cultivars were inoculated with ca. lO cfu/ml of the six strains. Two Australian strains (now ICMP 13298 and 13299) infected all twelve apple and all three pear cultivars inoculated. The third (now ICMP 13293) infected three of twelve apple but all three pear cultivars inoculated. In general, the virulence of the latter Australian strain, as judged by percent lesion length, was less than that of the first two Australian strains and was similar to that of one New Zealand strain, and strain Ea321. Our results support reports that fire blight occurred in Australia, and indicate that the Australian strains are similar to Maloideae-infecting strains of E. amylovora analyzed previously from North America, Europe and the Middle East.

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Kim JF, Garr ER, Bauer DW, Beer SV, Gustafson HL, Momol EA et al. Analysis of three bacterial strains isolated from symptomatic plants in Australia. In Momol MT, Saygili H, editors, VIII International Workshop on Fire Blight. 1999. p. 149-154. (Acta Horticulturae).