CW STED nanoscopy using illumination of continuous wave is considerably easy and less expensive to construct compared with the pulsed STED nanoscopy. In this study, to improve the resolution of CW STED nanoscopy, we analyzed the imaging characteristics of CW STED nanoscopy by amplitude modulation of incident light flux of the excitation beam illumination considering the polarization state and geometry of the pupil mask for amplitude modulation. We analyzed the imaging characteristics of STED nanoscopy by applying the characteristic, which shows an extremely confined electric field in transverse direction when the light waves with high spatial frequencies and with the same polarization direction are diffracted and interfered in the focal region. By applying linearly polarized illumination and the mixed-shaped aperture composed of the bow tie-shaped blocking area and the circular blocking aperture area, we analyzed that imaging resolution can be enhanced above 20% higher than the resolution of the conventional CW STED nanoscopy.
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics