Analysis on improvement in resolution by excitation beam modulation in stimulated emission depletion nanoscopy

Geon Lim, No Cheol Park, Wan Chin Kim

Research output: Contribution to journalArticle

Abstract

CW STED nanoscopy using illumination of continuous wave is considerably easy and less expensive to construct compared with the pulsed STED nanoscopy. In this study, to improve the resolution of CW STED nanoscopy, we analyzed the imaging characteristics of CW STED nanoscopy by amplitude modulation of incident light flux of the excitation beam illumination considering the polarization state and geometry of the pupil mask for amplitude modulation. We analyzed the imaging characteristics of STED nanoscopy by applying the characteristic, which shows an extremely confined electric field in transverse direction when the light waves with high spatial frequencies and with the same polarization direction are diffracted and interfered in the focal region. By applying linearly polarized illumination and the mixed-shaped aperture composed of the bow tie-shaped blocking area and the circular blocking aperture area, we analyzed that imaging resolution can be enhanced above 20% higher than the resolution of the conventional CW STED nanoscopy.

Original languageEnglish
Pages (from-to)512-521
Number of pages10
JournalOptical Review
Volume26
Issue number5
DOIs
Publication statusPublished - 2019 Oct 1

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stimulated emission
depletion
illumination
modulation
apertures
excitation
bows
polarization
pupils
continuous radiation
masks
electric fields
geometry

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

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abstract = "CW STED nanoscopy using illumination of continuous wave is considerably easy and less expensive to construct compared with the pulsed STED nanoscopy. In this study, to improve the resolution of CW STED nanoscopy, we analyzed the imaging characteristics of CW STED nanoscopy by amplitude modulation of incident light flux of the excitation beam illumination considering the polarization state and geometry of the pupil mask for amplitude modulation. We analyzed the imaging characteristics of STED nanoscopy by applying the characteristic, which shows an extremely confined electric field in transverse direction when the light waves with high spatial frequencies and with the same polarization direction are diffracted and interfered in the focal region. By applying linearly polarized illumination and the mixed-shaped aperture composed of the bow tie-shaped blocking area and the circular blocking aperture area, we analyzed that imaging resolution can be enhanced above 20{\%} higher than the resolution of the conventional CW STED nanoscopy.",
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Analysis on improvement in resolution by excitation beam modulation in stimulated emission depletion nanoscopy. / Lim, Geon; Park, No Cheol; Kim, Wan Chin.

In: Optical Review, Vol. 26, No. 5, 01.10.2019, p. 512-521.

Research output: Contribution to journalArticle

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