Anchored protease-activatable polymersomes for molecular diagnostics of metastatic cancer cells

Hyun Ouk Kim, Jong Woo Lim, Jihye Choi, Hwunjae Lee, Hye Young Son, Jihye Kim, Geunseon Park, Haejin Chun, Daesub Song, yongmin Huh, Seungjoo Haam

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Real-time quantitative and qualitative analyses of metastasis-associated proteases are critical for precise diagnosis and novel therapeutic treatment of advanced cancers. However, conventional methods based on DNA, peptides, and proteins require sophisticated chemistry and additional processes to expose detection moieties, and they lack elements of temporal control, which limit their applicability. We designed unique protease-activatable polymersomes (PeptiSomes) for high sensitivity, in situ quantitative analysis of activating membrane-type 1 matrix metalloproteinases (MT1-MMP, MMP14). To do this, we first synthesized an amphiphilic block polymer-peptide and a copolypeptide based on mPEG-b-pLeu and MT1-peptide-b-pLeu, respectively. Amphiphilic self-assembled PeptiSomes in water were capable of disassembling and releasing the encapsulated self-quenched fluorescence dye (calcein) via enzymatic activation by MT1-MMP. Our PeptiSome system may potentially prevent the initiation and progression of cancer metastasis. Furthermore, the PeptiSome approach described here is likely to facilitate the development of rapid protease assay techniques and further extend the role of proteases as metastasis indicators and therapeutic targets.

Original languageEnglish
Pages (from-to)9571-9578
Number of pages8
JournalJournal of Materials Chemistry B
Volume5
Issue number48
DOIs
Publication statusPublished - 2017 Jan 1

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Matrix Metalloproteinase 14
Peptide Hydrolases
Cells
Peptides
Block copolymers
Assays
DNA
Coloring Agents
Dyes
Fluorescence
Chemical activation
Proteins
Membranes
Water
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Kim, Hyun Ouk ; Lim, Jong Woo ; Choi, Jihye ; Lee, Hwunjae ; Son, Hye Young ; Kim, Jihye ; Park, Geunseon ; Chun, Haejin ; Song, Daesub ; Huh, yongmin ; Haam, Seungjoo. / Anchored protease-activatable polymersomes for molecular diagnostics of metastatic cancer cells. In: Journal of Materials Chemistry B. 2017 ; Vol. 5, No. 48. pp. 9571-9578.
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abstract = "Real-time quantitative and qualitative analyses of metastasis-associated proteases are critical for precise diagnosis and novel therapeutic treatment of advanced cancers. However, conventional methods based on DNA, peptides, and proteins require sophisticated chemistry and additional processes to expose detection moieties, and they lack elements of temporal control, which limit their applicability. We designed unique protease-activatable polymersomes (PeptiSomes) for high sensitivity, in situ quantitative analysis of activating membrane-type 1 matrix metalloproteinases (MT1-MMP, MMP14). To do this, we first synthesized an amphiphilic block polymer-peptide and a copolypeptide based on mPEG-b-pLeu and MT1-peptide-b-pLeu, respectively. Amphiphilic self-assembled PeptiSomes in water were capable of disassembling and releasing the encapsulated self-quenched fluorescence dye (calcein) via enzymatic activation by MT1-MMP. Our PeptiSome system may potentially prevent the initiation and progression of cancer metastasis. Furthermore, the PeptiSome approach described here is likely to facilitate the development of rapid protease assay techniques and further extend the role of proteases as metastasis indicators and therapeutic targets.",
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Kim, HO, Lim, JW, Choi, J, Lee, H, Son, HY, Kim, J, Park, G, Chun, H, Song, D, Huh, Y & Haam, S 2017, 'Anchored protease-activatable polymersomes for molecular diagnostics of metastatic cancer cells', Journal of Materials Chemistry B, vol. 5, no. 48, pp. 9571-9578. https://doi.org/10.1039/c7tb01675a

Anchored protease-activatable polymersomes for molecular diagnostics of metastatic cancer cells. / Kim, Hyun Ouk; Lim, Jong Woo; Choi, Jihye; Lee, Hwunjae; Son, Hye Young; Kim, Jihye; Park, Geunseon; Chun, Haejin; Song, Daesub; Huh, yongmin; Haam, Seungjoo.

In: Journal of Materials Chemistry B, Vol. 5, No. 48, 01.01.2017, p. 9571-9578.

Research output: Contribution to journalArticle

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AU - Kim, Hyun Ouk

AU - Lim, Jong Woo

AU - Choi, Jihye

AU - Lee, Hwunjae

AU - Son, Hye Young

AU - Kim, Jihye

AU - Park, Geunseon

AU - Chun, Haejin

AU - Song, Daesub

AU - Huh, yongmin

AU - Haam, Seungjoo

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