And glucose consumption in adipose-derived stem cells

Sireewan Kaewsuwan, Anuchit Plubrukarn, Maleeruk Utsintong, Seok Ho Kim, Jin Hyun Jeong, Jin Gu Cho, Sang Gyu Park, Jong Hyuk Sung

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5 Citations (Scopus)

Abstract

Interruptin B has been isolated from Cyclosorus terminans, however, its pharamcological effect has not been fully identified. In the present study, the effects of interruptin B, from C. terminans, on brown adipocyte differentiation and glucose uptake in adipose-derived stem cells (ASCs) were investigated. The results revealed that interruptin B dose-dependently enhanced the adipogenic differentiation of ASCs, with an induction in the mRNA expression levels of peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ. In addition, interruptin B efficiently increased the number and the membrane potential of mitochondria and upregulated the mRNA expression levels of uncoupling protein (UCP)-1 and cyclooxygenase (COX)-2, which are all predominantly expressed in brown adipocytes. Interruptin B increased glucose consumption in differentiated ASCs, accompanied by the upregulation in the mRNA expression levels of glucose transporter (GLUT)-1 and GLUT-4. The computational analysis of molecular docking, a luciferase reporter assay and surface plasmon resonance confirmed the marked binding affinity of interruptin B to PPAR-α and PPAR-γ (KD values of 5.32 and 0.10 μm, respectively). To the best of our knowledge, the present study is the first report to show the stimulatory effects of interruptin B on brown adipocyte differentiation and glucose uptake in ASCs, through its role as a dual PPAR-α and PPAR-γ ligand. Therefore, interruptin B could be further developed as a therapeutic agent for the treatment of diabetes.

Original languageEnglish
Pages (from-to)2078-2086
Number of pages9
JournalMolecular Medicine Reports
Volume13
Issue number3
DOIs
Publication statusPublished - 2016 Mar

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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  • Cite this

    Kaewsuwan, S., Plubrukarn, A., Utsintong, M., Kim, S. H., Jeong, J. H., Cho, J. G., Park, S. G., & Sung, J. H. (2016). And glucose consumption in adipose-derived stem cells. Molecular Medicine Reports, 13(3), 2078-2086. https://doi.org/10.3892/mmr.2016.4758