Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease

Dong Ik Kim, Mi Jung Kim, Jin Hyun Joh, Sung Wook Shin, Young Soo Do, Ji Young Moon, Na Ri Kim, Joung Eun Lim, Ae Kyeong Kim, Hyun Seon Eo, Byung Soo Kim, Seung-Woo Cho, Seung Hye Yang, Chan Jeoung Park, Jong Sup Shim

Research output: Contribution to journalArticle

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Abstract

We hypothesized that angiogenesis can be triggered by autologous whole bone marrow stem cell transplantation. Twenty-seven patients (34 lower limbs) with Buerger's disease, who were not candidates for surgical revascularization or radiologic intervention, were enrolled in this study. Six sites of the tibia bone were fenestrated using a 2.5-mm-diameter screw under fluoroscopic guidance. Clinical status and outcome were determined using the "Recommended Standards for Reports." To mobilize endothelial progenitor cells (EPCs) from bone marrow, recombinant human granulocyte colony-stimulating factor (r-HuG-CSF) was injected subcutaneously as a dose of 75 μg, preoperatively. During the follow-up period (19.1 ± 3.5 months), one limb showed a markedly improved outcome (+3), and 26 limbs showed a moderately improved outcome (+2). Thirteen limbs among 17 limbs with nonhealing ulcers healed. Postoperative angiograms were obtained for 22 limbs. Two limbs showed marked (+3), five limbs moderate (+2), and nine limbs slight (+1) collateral development. However, six limbs showed no collateral development (0). Peripheral blood and bone marrow samples were analyzed for CD34 and CD133 molecules to enumerate potential EPCs, and EPC numbers were found to be increased in peripheral blood and in bone marrow after r-HuG-CSF injection. In conclusion, the transplantation of autologous whole BMCs by fenestration of the tibia bone represents a simple, safe, and effective means of inducing therapeutic angiogenesis in patients with Buerger's disease.

Original languageEnglish
Pages (from-to)1194-1200
Number of pages7
JournalStem Cells
Volume24
Issue number5
DOIs
Publication statusPublished - 2006 May 1

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Thromboangiitis Obliterans
Stem Cell Transplantation
Bone Marrow Transplantation
Extremities
Bone Marrow
Granulocyte Colony-Stimulating Factor
Tibia
Bone and Bones
Autologous Transplantation
Ulcer
Lower Extremity
Angiography
Cell Count

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Kim, D. I., Kim, M. J., Joh, J. H., Shin, S. W., Do, Y. S., Moon, J. Y., ... Shim, J. S. (2006). Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease. Stem Cells, 24(5), 1194-1200. https://doi.org/10.1634/stemcells.2005-0349
Kim, Dong Ik ; Kim, Mi Jung ; Joh, Jin Hyun ; Shin, Sung Wook ; Do, Young Soo ; Moon, Ji Young ; Kim, Na Ri ; Lim, Joung Eun ; Kim, Ae Kyeong ; Eo, Hyun Seon ; Kim, Byung Soo ; Cho, Seung-Woo ; Yang, Seung Hye ; Park, Chan Jeoung ; Shim, Jong Sup. / Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease. In: Stem Cells. 2006 ; Vol. 24, No. 5. pp. 1194-1200.
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abstract = "We hypothesized that angiogenesis can be triggered by autologous whole bone marrow stem cell transplantation. Twenty-seven patients (34 lower limbs) with Buerger's disease, who were not candidates for surgical revascularization or radiologic intervention, were enrolled in this study. Six sites of the tibia bone were fenestrated using a 2.5-mm-diameter screw under fluoroscopic guidance. Clinical status and outcome were determined using the {"}Recommended Standards for Reports.{"} To mobilize endothelial progenitor cells (EPCs) from bone marrow, recombinant human granulocyte colony-stimulating factor (r-HuG-CSF) was injected subcutaneously as a dose of 75 μg, preoperatively. During the follow-up period (19.1 ± 3.5 months), one limb showed a markedly improved outcome (+3), and 26 limbs showed a moderately improved outcome (+2). Thirteen limbs among 17 limbs with nonhealing ulcers healed. Postoperative angiograms were obtained for 22 limbs. Two limbs showed marked (+3), five limbs moderate (+2), and nine limbs slight (+1) collateral development. However, six limbs showed no collateral development (0). Peripheral blood and bone marrow samples were analyzed for CD34 and CD133 molecules to enumerate potential EPCs, and EPC numbers were found to be increased in peripheral blood and in bone marrow after r-HuG-CSF injection. In conclusion, the transplantation of autologous whole BMCs by fenestration of the tibia bone represents a simple, safe, and effective means of inducing therapeutic angiogenesis in patients with Buerger's disease.",
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Kim, DI, Kim, MJ, Joh, JH, Shin, SW, Do, YS, Moon, JY, Kim, NR, Lim, JE, Kim, AK, Eo, HS, Kim, BS, Cho, S-W, Yang, SH, Park, CJ & Shim, JS 2006, 'Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease', Stem Cells, vol. 24, no. 5, pp. 1194-1200. https://doi.org/10.1634/stemcells.2005-0349

Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease. / Kim, Dong Ik; Kim, Mi Jung; Joh, Jin Hyun; Shin, Sung Wook; Do, Young Soo; Moon, Ji Young; Kim, Na Ri; Lim, Joung Eun; Kim, Ae Kyeong; Eo, Hyun Seon; Kim, Byung Soo; Cho, Seung-Woo; Yang, Seung Hye; Park, Chan Jeoung; Shim, Jong Sup.

In: Stem Cells, Vol. 24, No. 5, 01.05.2006, p. 1194-1200.

Research output: Contribution to journalArticle

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T1 - Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease

AU - Kim, Dong Ik

AU - Kim, Mi Jung

AU - Joh, Jin Hyun

AU - Shin, Sung Wook

AU - Do, Young Soo

AU - Moon, Ji Young

AU - Kim, Na Ri

AU - Lim, Joung Eun

AU - Kim, Ae Kyeong

AU - Eo, Hyun Seon

AU - Kim, Byung Soo

AU - Cho, Seung-Woo

AU - Yang, Seung Hye

AU - Park, Chan Jeoung

AU - Shim, Jong Sup

PY - 2006/5/1

Y1 - 2006/5/1

N2 - We hypothesized that angiogenesis can be triggered by autologous whole bone marrow stem cell transplantation. Twenty-seven patients (34 lower limbs) with Buerger's disease, who were not candidates for surgical revascularization or radiologic intervention, were enrolled in this study. Six sites of the tibia bone were fenestrated using a 2.5-mm-diameter screw under fluoroscopic guidance. Clinical status and outcome were determined using the "Recommended Standards for Reports." To mobilize endothelial progenitor cells (EPCs) from bone marrow, recombinant human granulocyte colony-stimulating factor (r-HuG-CSF) was injected subcutaneously as a dose of 75 μg, preoperatively. During the follow-up period (19.1 ± 3.5 months), one limb showed a markedly improved outcome (+3), and 26 limbs showed a moderately improved outcome (+2). Thirteen limbs among 17 limbs with nonhealing ulcers healed. Postoperative angiograms were obtained for 22 limbs. Two limbs showed marked (+3), five limbs moderate (+2), and nine limbs slight (+1) collateral development. However, six limbs showed no collateral development (0). Peripheral blood and bone marrow samples were analyzed for CD34 and CD133 molecules to enumerate potential EPCs, and EPC numbers were found to be increased in peripheral blood and in bone marrow after r-HuG-CSF injection. In conclusion, the transplantation of autologous whole BMCs by fenestration of the tibia bone represents a simple, safe, and effective means of inducing therapeutic angiogenesis in patients with Buerger's disease.

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