Anthranilate degradation by a cold-adapted Pseudomonas sp.

Dockyu Kim, Miyoun Yoo, Eungbin KIm, Soon Gyu Hong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An alpine soil bacterium Pseudomonas sp. strain PAMC 25931 was characterized as eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 5-30°C both for anthranilate (2-aminobenzoate) degradation and concomitant cell growth. Two degradative gene clusters (antABC and catBCA) were detected from a fosmid clone in the PAMC 25931 genomic library; each cluster was confirmed to be specifically induced by anthranilate. When expressed in Escherichia coli, the recombinant AntABC (anthranilate 1,2-dioxygenase, AntDO) converted anthranilate into catechol, exhibiting strict specificity toward anthranilate. Recombinant CatA (catechol 1,2-dioxygenase, C12O) from the organism was active over a broad temperature range (5-37°C). However, CatA rapidly lost the enzyme activity when incubated at above 25°C. For example, 1h-preincubation at 37°C resulted in 100% loss of enzyme activity, while a counterpart from mesophilic Pseudomonas putida mt-2 did not show any negative effect on the initial enzyme activity. These results suggest that CatA is a new cold-adapted thermolabile enzyme, which might be a product through the adaptation process of PAMC 25931 to naturally cold environments and contribute to its ability to grow on anthranilate there.

Original languageEnglish
Pages (from-to)354-362
Number of pages9
JournalJournal of Basic Microbiology
Volume55
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1

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Pseudomonas
Enzymes
ortho-Aminobenzoates
Catechol 1,2-Dioxygenase
Pseudomonas putida
Temperature
Genomic Library
Multigene Family
Soil
Clone Cells
anthranilic acid
Escherichia coli
Bacteria
Growth

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

Cite this

Kim, Dockyu ; Yoo, Miyoun ; KIm, Eungbin ; Hong, Soon Gyu. / Anthranilate degradation by a cold-adapted Pseudomonas sp. In: Journal of Basic Microbiology. 2015 ; Vol. 55, No. 3. pp. 354-362.
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Anthranilate degradation by a cold-adapted Pseudomonas sp. / Kim, Dockyu; Yoo, Miyoun; KIm, Eungbin; Hong, Soon Gyu.

In: Journal of Basic Microbiology, Vol. 55, No. 3, 01.01.2015, p. 354-362.

Research output: Contribution to journalArticle

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