Abstract
Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 206-211 |
| Number of pages | 6 |
| Journal | Journal of Biochemistry and Molecular Biology |
| Volume | 34 |
| Issue number | 3 |
| Publication status | Published - 2001 May 31 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
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Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin. / Cho, Heeyeong; Kim, Woo Jean; Lee, Sae Woo; Kim, Young Mi; Choi, Eu Yul; Park, Yong Suk; Kwon, Young-Guen; Kim, Kyu Won.
In: Journal of Biochemistry and Molecular Biology, Vol. 34, No. 3, 31.05.2001, p. 206-211.Research output: Contribution to journal › Article
TY - JOUR
T1 - Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin
AU - Cho, Heeyeong
AU - Kim, Woo Jean
AU - Lee, Sae Woo
AU - Kim, Young Mi
AU - Choi, Eu Yul
AU - Park, Yong Suk
AU - Kwon, Young-Guen
AU - Kim, Kyu Won
PY - 2001/5/31
Y1 - 2001/5/31
N2 - Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.
AB - Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0035592827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035592827&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0035592827
VL - 34
SP - 206
EP - 211
JO - BMB Reports
JF - BMB Reports
SN - 1976-6696
IS - 3
ER -