Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin

Heeyeong Cho, Woo Jean Kim, Sae Woo Lee, Young Mi Kim, Eu Yul Choi, Yong Suk Park, Young-Guen Kwon, Kyu Won Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume34
Issue number3
Publication statusPublished - 2001 May 31

Fingerprint

Endostatins
Tumors
Chorioallantoic Membrane
Growth Inhibitors
Angiogenesis Inhibitors
Assays
Neoplasms
Collagen Type XVIII
Derivatives
Membranes
Endothelial cells
Human Umbilical Vein Endothelial Cells
Chick Embryo
Growth
Tretinoin
Bioactivity
Thymidine
Melanoma

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Cho, H., Kim, W. J., Lee, S. W., Kim, Y. M., Choi, E. Y., Park, Y. S., ... Kim, K. W. (2001). Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin. Journal of Biochemistry and Molecular Biology, 34(3), 206-211.
Cho, Heeyeong ; Kim, Woo Jean ; Lee, Sae Woo ; Kim, Young Mi ; Choi, Eu Yul ; Park, Yong Suk ; Kwon, Young-Guen ; Kim, Kyu Won. / Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin. In: Journal of Biochemistry and Molecular Biology. 2001 ; Vol. 34, No. 3. pp. 206-211.
@article{417e02b8427146db81646c040a7df248,
title = "Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin",
abstract = "Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71{\%}, which is comparative to a 80{\%} effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.",
author = "Heeyeong Cho and Kim, {Woo Jean} and Lee, {Sae Woo} and Kim, {Young Mi} and Choi, {Eu Yul} and Park, {Yong Suk} and Young-Guen Kwon and Kim, {Kyu Won}",
year = "2001",
month = "5",
day = "31",
language = "English",
volume = "34",
pages = "206--211",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "3",

}

Cho, H, Kim, WJ, Lee, SW, Kim, YM, Choi, EY, Park, YS, Kwon, Y-G & Kim, KW 2001, 'Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin', Journal of Biochemistry and Molecular Biology, vol. 34, no. 3, pp. 206-211.

Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin. / Cho, Heeyeong; Kim, Woo Jean; Lee, Sae Woo; Kim, Young Mi; Choi, Eu Yul; Park, Yong Suk; Kwon, Young-Guen; Kim, Kyu Won.

In: Journal of Biochemistry and Molecular Biology, Vol. 34, No. 3, 31.05.2001, p. 206-211.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin

AU - Cho, Heeyeong

AU - Kim, Woo Jean

AU - Lee, Sae Woo

AU - Kim, Young Mi

AU - Choi, Eu Yul

AU - Park, Yong Suk

AU - Kwon, Young-Guen

AU - Kim, Kyu Won

PY - 2001/5/31

Y1 - 2001/5/31

N2 - Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.

AB - Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0035592827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035592827&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0035592827

VL - 34

SP - 206

EP - 211

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 3

ER -

Cho H, Kim WJ, Lee SW, Kim YM, Choi EY, Park YS et al. Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin. Journal of Biochemistry and Molecular Biology. 2001 May 31;34(3):206-211.