Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin

Heeyeong Cho, Woo Jean Kim, Sae Woo Lee, Young Mi Kim, Eu Yul Choi, Yong Suk Park, Young Guen Kwon, Kyu Won Kim

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Abstract

Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [3H]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of 200 ng of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, 1 μg of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N- and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume34
Issue number3
Publication statusPublished - 2001 May 31

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Cho, H., Kim, W. J., Lee, S. W., Kim, Y. M., Choi, E. Y., Park, Y. S., Kwon, Y. G., & Kim, K. W. (2001). Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin. Journal of Biochemistry and Molecular Biology, 34(3), 206-211.