Anti-atherosclerotic vaccination against Porphyromonas gingivalis as a potential comparator of statin in mice

Hyun Su Ha, Tae Young Kim, Soo Jung Han, Hak Joon Sung, Kyoung Yul Seo, Jong Won Ha

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2 Citations (Scopus)


Background. Porphyromonas gingivalis (Pg) is an oral anaerobe which damages teeth and periodontal tissues. Its body infection is known to cause chronic inflammation, thereby inducing an early stage of atherosclerosis through humoral immune actions. Hence, vaccination by immunizing the proteins of P. gingivalis (Pg) post sonication with heating may prevent atherosclerosis. This study aimed to compare the effect of its vaccination with statin, which effectively prevents atherosclerosis by lowering lipids. Methods. The vaccine was produced by sonicating P. gingivalis through heating, and a total of 32 male APOE-/-mice (8-week old) were subjected Western diet for 8 weeks, in order to induce atherosclerosis in a physiological manner. Then, the mice were grouped to undergo four treatment conditions (i.e., no treatment, pitavastatin, vaccine, or pitavastatin with vaccine). Vaccination was conducted through nasal immunization and confirmed by a Pg-specific humoral immune reaction. Then, half of the mice in each group were orally injected with P. gingivalis for the next 5 weeks while the other half remained uninfected, generating a total of eight groups (n = 4/group). The mice were sacrificed at 3 weeks after the last injection. After harvesting the aorta, Oil Red O staining of en face was conducted with imaging and image analysis, and plaque formation was quantitatively determined. Results. Compared to no treatment, the vaccination through nasal immunization significantly reduced the atherosclerotic plaque sizes in APOE -/- mice under Western diet to the comparable level of statin group. When both vaccine and statin were used, no clear synergistic effect was observed as opposed to expectation. Conclusions. This study revealed that nasal immunization of heat shock P. gingivalis has a significant impact on the prevention of arteriosclerosis and acts as a potential comparator of statin.

Original languageEnglish
Article numbere11293
Publication statusPublished - 2021 Apr 20

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) (No. 2016M3A9E9941743), and Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science and ICT (2016M3A9E9941746), and this study was funded by the JW Pharmaceutical Corporation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Funding Information:
The following grant information was disclosed by the authors: National Research Foundation of Korea (NRF): 2016M3A9E9941743. Bio & Medical Technology Development Program of the National Research Foundation (NRF). Ministry of Science and ICT: 2016M3A9E9941746. JW Pharmaceutical Corporation.

Publisher Copyright:
© Copyright 2021 Ha et al.

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)


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