Anti-biofilm activity of xanthorrhizol isolated from Curcuma xanthorrhiza roxb. against bacterial biofilms formed by saliva and artificial multi-species oral strains

Yanti, Yaya Rukayadi, Kwanhyoung Lee, Sunghwa Han, Jae Kwan Hwang

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4 Citations (Scopus)


Xanthorrhizol, a sesquiterpene isolated from Curcuma xanthorrhiza Roxb., was used to investigate its effect on reducing the saliva and multi-species oral biofilms consisting of Streptococcus mutans, Streptococcus sanguis, and Actinomyces viscosus by anti-biofilm and confocal laser scanning microscopy (CLSM) assays. Xanthorrhizol exhibited significant antibiofilm activity in the dose- and time-dependent manners. Exposure to 2 and 5 μg/mL xanthorrhizol for 30 min remained <50% of saliva and multi-species biofilms formed for 24 hr. In addition, exposure to 10 μg/mL xanthorrhizol for 30 min reduced 65 and 77% of 24 hr saliva and multi-species oral biofilms, respectively. CLSM results visually demonstrated that xanthorrhizol reduced bacterial viability in the saliva and multi-species oral biofilms. These results suggest that C. xanthorrhiza Roxb. containing xanthorrhizol with strong anti-biofilm activity can be employed as a plant source for oral care functional foods.

Original languageEnglish
Pages (from-to)556-560
Number of pages5
JournalFood Science and Biotechnology
Issue number2
Publication statusPublished - 2009 Apr 18


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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