Anti-histone acetyltransferase activity from allspice extracts inhibits androgen receptor-dependent prostate cancer cell growth

Yoo Hyun Lee, Soon Won Hong, Woojin Jun, Hong Yon Cho, Han Cheon Kim, Myung Gu Jung, Jiemin Wong, Ha Il Kim, Chang Hoon Kim, Ho Geun Yoon

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Histone acetylation depends on the activity of two enzyme families, histone acetyltransferase (HAT) and deacetylase (HDAC). In this study, we screened various plant extracts to find potent HAT inhibitors. Hot water extracts of allspice inhibited HAT activity, especially p300 and CBP (40% at 100 μg/ml). The mRNA levels of two androgen receptor (AR) regulated genes, PSA and TSC22, decreased with allspice treatment (100 μg/ml). Importantly, in IP western analysis, AR acetylation was dramatically decreased by allspice treatment. Furthermore, chromatin immunoprecipitation indicated that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed. Finally, allspice treatment reduced the growth of human prostate cancer cells, LNCaP (50% growth inhibition at 200 μg/ml). Taken together, our data indicate that the potent HAT inhibitory activity of allspice reduced AR and histone acetylation and led to decreased transcription of AR target genes, resulting in inhibition of prostate cancer cell growth.

Original languageEnglish
Pages (from-to)2712-2719
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number11
DOIs
Publication statusPublished - 2007 Nov 30

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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