Anti-IgM induces up-regulation and tyrosine-phosphorylation of heterogeneous nuclear ribonucleoprotein K proteins (hnRNP K) in a Ramos B cell line

Hye Kyung Jeon, Jeong Hun Ahn, Jongseon Choe, Jeon Han Park, Tae Ho Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Heterogeneous nuclear ribonucleoprotein K protein (hnRNP K) has diverse molecular partners implicated in signal transduction pathways, and is tyrosine-phosphorylated in response to growth factors and oxidative stress. Among the structurally distinct domains of hnRNP K, an SH3-binding domain (SH3BD) has been known to promote the association of SH3-containing tyrosine kinases and protooncoprotein Vav, which are involved in B cell receptor (BCR) signalling. In this study, we analyzed proteins of Ramos B cell line that are altered upon BCR activation with anti-IgM antibody, revealing that a certain hnRNP K isoform is up-regulated in response to anti-IgM treatment. We also showed that hnRNP K is tyrosine-phosphorylated after BCR ligation. HnRNP K lacking the SH3BD is shown not to interact with phosphorylated Vav, and Ramos cells stably expressing this mutant protein are less susceptible to anti-IgM-induced apoptosis, indicating that hnRNP K is coupled to BCR-mediated signalling and its SH3BD is required for proper signal propagation. Our results provide the first evidence that hnRNP K is involved in BCR signalling pathway.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
JournalImmunology Letters
Volume98
Issue number2
DOIs
Publication statusPublished - 2005 May 15

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Heterogeneous-Nuclear Ribonucleoprotein K
Tyrosine
B-Lymphocytes
Up-Regulation
Phosphorylation
Cell Line
src Homology Domains
Proteins
Mutant Proteins
Protein Binding
Protein-Tyrosine Kinases
Ligation
anti-IgM
Anti-Idiotypic Antibodies
Signal Transduction
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Oxidative Stress
Apoptosis

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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title = "Anti-IgM induces up-regulation and tyrosine-phosphorylation of heterogeneous nuclear ribonucleoprotein K proteins (hnRNP K) in a Ramos B cell line",
abstract = "Heterogeneous nuclear ribonucleoprotein K protein (hnRNP K) has diverse molecular partners implicated in signal transduction pathways, and is tyrosine-phosphorylated in response to growth factors and oxidative stress. Among the structurally distinct domains of hnRNP K, an SH3-binding domain (SH3BD) has been known to promote the association of SH3-containing tyrosine kinases and protooncoprotein Vav, which are involved in B cell receptor (BCR) signalling. In this study, we analyzed proteins of Ramos B cell line that are altered upon BCR activation with anti-IgM antibody, revealing that a certain hnRNP K isoform is up-regulated in response to anti-IgM treatment. We also showed that hnRNP K is tyrosine-phosphorylated after BCR ligation. HnRNP K lacking the SH3BD is shown not to interact with phosphorylated Vav, and Ramos cells stably expressing this mutant protein are less susceptible to anti-IgM-induced apoptosis, indicating that hnRNP K is coupled to BCR-mediated signalling and its SH3BD is required for proper signal propagation. Our results provide the first evidence that hnRNP K is involved in BCR signalling pathway.",
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Anti-IgM induces up-regulation and tyrosine-phosphorylation of heterogeneous nuclear ribonucleoprotein K proteins (hnRNP K) in a Ramos B cell line. / Jeon, Hye Kyung; Ahn, Jeong Hun; Choe, Jongseon; Park, Jeon Han; Lee, Tae Ho.

In: Immunology Letters, Vol. 98, No. 2, 15.05.2005, p. 303-310.

Research output: Contribution to journalArticle

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AU - Lee, Tae Ho

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