Anti-inflammatory activity of mulberry leaf extract through inhibition of NF-κB

Eunkyo Park, Seung-Min Lee, Jung eun Lee, Jung Hyun Kim

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Mulberry leaf has been traditionally used to treat chronic diseases such as diabetes and cancer. The effect and mechanism of mulberry leaf extract (MLE) in LPS-induced activation of macrophage was investigated by the levels of production of proinflammatory mediators and cytokines, and their transcriptions. MLE effectively decreased proinflammatory mediators and cytokines production. Their expression levels were decreased at both transcript and protein levels by the pretreatment of MLE. Since all these genes are transcribed by NF-κB and MAPK, their activities were also examined. NF-κB activity was proved to be reduced by the preincubation with MLE through inhibition of IκBα phosphorylation. However, MLE-treatment had no effect on phosphorylation levels of MAPK. There results suggest that MLE can be used as an anti-inflammatory agent to inhibit NF-κB-mediated inflammatory response. In addition, our data support the idea that MLE can inhibit the activities of proinflammatory mediators and cytokines to ameliorate the disease conditions.

Original languageEnglish
Pages (from-to)178-186
Number of pages9
JournalJournal of Functional Foods
Volume5
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Morus
mulberries
anti-inflammatory activity
leaf extracts
Anti-Inflammatory Agents
cytokines
Cytokines
phosphorylation
Phosphorylation
anti-inflammatory agents
macrophage activation
Macrophage Activation
chronic diseases
diabetes
Chronic Disease
pretreatment
transcription (genetics)
inflammation
neoplasms

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Park, Eunkyo ; Lee, Seung-Min ; Lee, Jung eun ; Kim, Jung Hyun. / Anti-inflammatory activity of mulberry leaf extract through inhibition of NF-κB. In: Journal of Functional Foods. 2013 ; Vol. 5, No. 1. pp. 178-186.
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Anti-inflammatory activity of mulberry leaf extract through inhibition of NF-κB. / Park, Eunkyo; Lee, Seung-Min; Lee, Jung eun; Kim, Jung Hyun.

In: Journal of Functional Foods, Vol. 5, No. 1, 01.01.2013, p. 178-186.

Research output: Contribution to journalArticle

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AB - Mulberry leaf has been traditionally used to treat chronic diseases such as diabetes and cancer. The effect and mechanism of mulberry leaf extract (MLE) in LPS-induced activation of macrophage was investigated by the levels of production of proinflammatory mediators and cytokines, and their transcriptions. MLE effectively decreased proinflammatory mediators and cytokines production. Their expression levels were decreased at both transcript and protein levels by the pretreatment of MLE. Since all these genes are transcribed by NF-κB and MAPK, their activities were also examined. NF-κB activity was proved to be reduced by the preincubation with MLE through inhibition of IκBα phosphorylation. However, MLE-treatment had no effect on phosphorylation levels of MAPK. There results suggest that MLE can be used as an anti-inflammatory agent to inhibit NF-κB-mediated inflammatory response. In addition, our data support the idea that MLE can inhibit the activities of proinflammatory mediators and cytokines to ameliorate the disease conditions.

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